ArticleInfection and immunity2003
Two-component systems in Haemophilus influenzae: a regulatory role for ArcA in serum resistance.
Article in Infection and immunity, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 57 citations in OpenAlex.
- Two-Component Systems inVeterinary sciences · 2025Review
- Determinants of bacterial survival and proliferation in blood.FEMS microbiology reviews · 2024Review
- Metal Messengers: Communication in the Bacterial World through Transition-Metal-Sensing Two-Component Systems.Biochemistry · 2023Review
- The ArcAB Two-Component System: Function in Metabolism, Redox Control, and Infection.Microbiology and molecular biology reviews : MMBR · 2022Review
- The effect of ArcA on the growth, motility, biofilm formation, and virulence of Plesiomonas shigelloides.BMC microbiology · 2021Article
- Learning from -omics strategies applied to uncoverComputational and structural biotechnology journal · 2021Review
- An ArcA-Modulated Small RNA in PathogenicFrontiers in microbiology · 2020Article
- Article
- Reprogramming of Yersinia from virulent to persistent mode revealed by complex in vivo RNA-seq analysis.PLoS pathogens · 2015Article
- Comparative analyses of proteins from Haemophilus influenzae biofilm and planktonic populations using metabolic labeling and mass spectrometry.BMC microbiology · 2014Article
- Characterization of lactate utilization and its implication on the physiology of Haemophilus influenzae.International journal of medical microbiology : IJMM · 2014Article
- Metabolic versatility in Haemophilus influenzae: a metabolomic and genomic analysis.Frontiers in microbiology · 2014Article
- "Affect of anaerobiosis on the antibiotic susceptibility of H. influenzae".BMC research notes · 2013Article
- Article
- Characterization of a ferrous iron-responsive two-component system in nontypeable Haemophilus influenzae.Journal of bacteriology · 2012Article
- Novel screening assay for in vivo selection of Klebsiella pneumoniae genes promoting gastrointestinal colonisation.BMC microbiology · 2012Article
- Haemophilus influenzae and oxidative stress.Frontiers in cellular and infection microbiology · 2012Review
- Genome-scale approaches to identify genes essential for Haemophilus influenzae pathogenesis.Frontiers in cellular and infection microbiology · 2012Review
- ArcA-regulated glycosyltransferase lic2B promotes complement evasion and pathogenesis of nontypeable Haemophilus influenzae.Infection and immunity · 2011Article
- Resistance of Haemophilus influenzae to reactive nitrogen donors and gamma interferon-stimulated macrophages requires the formate-dependent nitrite reductase regulator-activated ytfE gene.Infection and immunity · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Knockout mutations were constructed in the arcA gene of a virulent type b strain of Haemophilus influenzae, and the behavior of the resulting mutants was investigated in a number of conditions that mimicked distinct steps in the natural infection pathway. In arcA mutants, synthesis of capsule and lipooligosaccharide (LOS) and growth in synthetic media were unaltered compared to synthesis of capsule and LOS and growth in synthetic media in the wild-type H. influenzae type b parent strain. However, the virulence of the arcA mutants for BALB/c mice was significantly reduced. Upon exposure to human blood or serum, the arcA mutants showed markedly reduced survival compared with the survival of its wild-type parent. Serum resistance could be fully restored by complementation in cis with the H. influenzae arcA gene but not by complementation in cis with the homologous gene from Escherichia coli. The proteomes of wild-type and mutant bacteria were markedly different, especially under anaerobic conditions, underscoring the global regulatory role of ArcAB in H. influenzae. Evaluation of antibody titers and classical complement activities in various serum samples pointed to complement-mediated bactericidal activity as the factor that distinguishes between the arcA mutant and wild-type phenotypes. Comparative analysis of the membrane fractions of the arcA mutants and the wild-type strain revealed several ArcA-regulated proteins, some of which may be implicated in the serum hypersensitivity phenotype.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.