ArticleBiophysical journal2002
Picosecond-hetero-FRET microscopy to probe protein-protein interactions in live cells.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 131 citations in OpenAlex.
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- Advantages and Limitations of Fluorescence Lifetime Measurements Using Single-Photon Avalanche Diode (SPAD) Array Detector: A Comprehensive Theoretical and Experimental Study.Sensors (Basel, Switzerland) · 2022Article
- An apparent core/shell architecture of polyQ aggregates in the aging Caenorhabditis elegans neuron.Protein science : a publication of the Protein Society · 2021Article
- Fluorescence-based techniques for the detection of the oligomeric status of proteins: implication in amyloidogenic diseases.European biophysics journal : EBJ · 2021Review
- Investigating tunneling nanotubes in ovarian cancer based on two-photon excitation FLIM-FRET.Biomedical optics express · 2021Article
- Wide-field time-gated SPAD imager for phasor-based FLIM applications.Methods and applications in fluorescence · 2020Article
- Spinal Inhibition of GABAB Receptors by the Extracellular Matrix Protein Fibulin-2 in Neuropathic Rats.Frontiers in cellular neuroscience · 2020Article
- An invisible private 2D barcode design and implementation with tunable fluorescent nanoparticles.RSC advances · 2019Article
- Investigating supramolecular systems using Förster resonance energy transfer.Chemical Society reviews · 2018Review
- Disentanglement of excited-state dynamics with implications for FRET measurements: two-dimensional electronic spectroscopy of a BODIPY-functionalized cavitand.Chemical science · 2018Article
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- PucC and LhaA direct efficient assembly of the light-harvesting complexes in Rhodobacter sphaeroides.Molecular microbiology · 2016Article
- X-Ray Crystal Structure and Properties of Phanta, a Weakly Fluorescent Photochromic GFP-Like Protein.PloS one · 2015Article
- Application of phasor plot and autofluorescence correction for study of heterogeneous cell population.Journal of biomedical optics · 2014Article
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- Grb14 is a negative regulator of CEACAM3-mediated phagocytosis of pathogenic bacteria.The Journal of biological chemistry · 2012Article
- Fluorescence lifetime imaging microscopy of intracellular glucose dynamics.Journal of diabetes science and technology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
By using a novel time- and space-correlated single-photon counting detector, we show that fluorescence resonance energy transfer (FRET) between cyan fluorescent protein (CFP) and yellow fluorescent protein (YFP) fused to herpes simplex virus thymidine kinase (TK) monomers can be used to reveal homodimerization of TK in the nucleus and cytoplasm of live cells. However, the quantification of energy transfer was limited by the intrinsic biexponential fluorescence decay of the donor CFP (lifetimes of 1.3 +/- 0.2 ns and 3.8 +/- 0.4 ns) and by the possibility of homodimer formation between two TK-CFP. In contrast, the heterodimerization of the transcriptional factor NF-E2 in the nucleus of live cells was quantified from the analysis of the fluorescence decays of GFP in terms of 1) FRET efficiency between GFP and DsRed chromophores fused to p45 and MafG, respectively, the two subunits of NF-E2 (which corresponds to an interchromophoric distance of 39 +/- 1 A); and 2) fractions of GFP-p45 bound to DsRed-MafG (constant in the nucleus, varying in the range of 20% to 70% from cell to cell). The picosecond resolution of the fluorescence kinetics allowed us to discriminate between very short lifetimes of immature green species of DsRed-MafG and that of GFP-p45 involved in FRET with DsRed-MafG.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.