ArticleBiophysical journal2002
Bidirectional control of sphingomyelinase activity and surface topography in lipid monolayers.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 80 citations in OpenAlex.
- The Effect of Ceramide Ratio on the Membrane Curvature of Mimetic Models of Matrix Vesicles.ACS physical chemistry Au · 2025Article
- Ceramide and the membrane-fusion activity of LC3/GABARAP autophagy proteins.Cellular and molecular life sciences : CMLS · 2025Article
- Fifty years of biophysics in Argentina.Biophysical reviews · 2023Article
- Review
- The many faces (and phases) of ceramide and sphingomyelin II - binary mixtures.Biophysical reviews · 2017Review
- Massive glycosaminoglycan-dependent entry of Trp-containing cell-penetrating peptides induced by exogenous sphingomyelinase or cholesterol depletion.Cellular and molecular life sciences : CMLS · 2015Article
- Effects of sphingomyelin headgroup size on interactions with ceramide.Biophysical journal · 2013Article
- Insights into sphingolipid miscibility: separate observation of sphingomyelin and ceramide N-acyl chain melting.Biophysical journal · 2012Article
- Effects of sphingosine 2N- and 3O-methylation on palmitoyl ceramide properties in bilayer membranes.Biophysical journal · 2011Article
- Cholesterol displaces palmitoylceramide from its tight packing with palmitoylsphingomyelin in the absence of a liquid-disordered phase.Biophysical journal · 2010Article
- Coexistence of immiscible mixtures of palmitoylsphingomyelin and palmitoylceramide in monolayers and bilayers.Biophysical journal · 2009Article
- Lipid raft composition modulates sphingomyelinase activity and ceramide-induced membrane physical alterations.Biophysical journal · 2009Article
- Sphingomyelinase-induced domain shape relaxation driven by out-of-equilibrium changes of composition.Biophysical journal · 2009Article
- Protein-induced surface structuring in myelin membrane monolayers.Biophysical journal · 2007Article
- Recent advances in the immunobiology of ceramide.Experimental and molecular pathology · 2007Review
- Interaction of the macrolide antibiotic azithromycin with lipid bilayers: effect on membrane organization, fluidity, and permeability.Pharmaceutical research · 2005Article
- Shape transitions and lattice structuring of ceramide-enriched domains generated by sphingomyelinase in lipid monolayers.Biophysical journal · 2005Article
- Asymmetric addition of ceramides but not dihydroceramides promotes transbilayer (flip-flop) lipid motion in membranes.Biophysical journal · 2005Article
- A lipid-specific toxin reveals heterogeneity of sphingomyelin-containing membranes.Biophysical journal · 2004Article
- A comparison of trabecular meshwork sphingolipids and ceramides of ocular normotensive and hypertensive states of DBA/2J mice.Journal of ocular pharmacology and therapeutics : the official journal of the Association for Ocular Pharmacology and TherapeuticsArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid lateral organization is increasingly found to modulate membrane-bound enzymes. We followed in real time the reaction course of sphingomyelin (SM) degradation by Bacillus cereus sphingomyelinase (SMase) of lipid monolayers by epifluorescence microscopy. There is evidence that formation of ceramide (Cer), a lipid second messenger, drives structural reorganization of membrane lipids. Our results provide visual evidence that SMase activity initially alters surface topography by inducing phase separation into condensed (Cer-enriched) and expanded (SM-enriched) domains. The Cer-enriched phase grows steadily as the reaction proceeds at a constant rate. The surface topography derived from the SMase-driven reaction was compared with, and found to differ from, that of premixed SM/Cer monolayers of the same lipid composition, indicating that substantial information content is stored depending on the manner in which the surface was generated. The long-range topographic changes feed back on the kinetics of Smase, and the onset of condensed-phase percolation is temporally correlated with a rapid drop of reaction rate. These observations reveal a bidirectional influence and communication between effects taking place at the local molecular level and the supramolecular organization. The results suggest a novel biocatalytic-topographic mechanism in which a surface enzymatic activity can influence the function of amphitropic proteins important for cell function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.