ArticleBiophysical journal2002
Obstructed diffusion in phase-separated supported lipid bilayers: a combined atomic force microscopy and fluorescence recovery after photobleaching approach.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 140 citations in OpenAlex.
- Model Lipid Bilayers as Platforms for Replicating Bacterial Membranes: A Study of Dynamics and Structural Heterogeneity.The journal of physical chemistry. B · 2026Article
- Experimentally Probing the Effect of Confinement Geometry on Lipid Diffusion.The journal of physical chemistry. B · 2024Article
- X-ray reflectivity study of the heat shock protein Hsp70 interaction with an artificial cell membrane model.Scientific reports · 2023Article
- Photomanipulation of Minimal Synthetic Cells: Area Increase, Softening, and Interleaflet Coupling of Membrane Models Doped with Azobenzene-Lipid Photoswitches.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- PDMS as a Substrate for Lipid Bilayers.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Comparative Study of Lipid- and Polymer-Supported Membranes Obtained by Vesicle Fusion.Langmuir : the ACS journal of surfaces and colloids · 2022Article
- Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide.Biomolecules · 2020Article
- To Hop or not to Hop: Exceptions in the FCS Diffusion Law.Biophysical journal · 2020Article
- Alteration of Membrane Physicochemical Properties by Two Factors for Membrane Protein Integration.Biophysical journal · 2019Article
- Real-time visualization of perforin nanopore assembly.Nature nanotechnology · 2017Article
- Dynamics of Crowding-Induced Mixing in Phase Separated Lipid Bilayers.The journal of physical chemistry. B · 2016Article
- Lipid14: The Amber Lipid Force Field.Journal of chemical theory and computation · 2014Article
- Bayesian total internal reflection fluorescence correlation spectroscopy reveals hIAPP-induced plasma membrane domain organization in live cells.Biophysical journal · 2014Article
- Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths.Faraday discussions · 2013Article
- Wanted: a positive control for anomalous subdiffusion.Biophysical journal · 2012Review
- Localized surface plasmon microscopy of submicron domain structures of mixed lipid bilayers.Biomedical optics express · 2012Article
- Derivation and systematic validation of a refined all-atom force field for phosphatidylcholine lipids.The journal of physical chemistry. B · 2012Article
- Article
- Electrostatic field effects on membrane domain segregation and on lateral diffusion.Biophysical reviews · 2011Review
- Membrane restructuring by phospholipase A2 is regulated by the presence of lipid domains.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteins and other macromolecules are believed to hinder molecular lateral diffusion in cellular membranes. We have constructed a well-characterized model system to better understand how obstacles in lipid bilayers obstruct diffusion. Fluorescence recovery after photobleaching was used to measure the lateral diffusion coefficient in single supported bilayers composed of mixtures of 1,2-dilauroylphosphotidylcholine (DLPC) and 1,2-distearoylphosphotidylcholine (DSPC). Because these lipids are immiscible and phase separate at room temperature, a novel quenching technique allowed us to construct fluid DLPC bilayers containing small disk-shaped gel-phase DSPC domains that acted as obstacles to lateral diffusion. Our experimental setup enabled us to analyze the same samples with atomic force microscopy and exactly characterize the size, shape, and number of gel-phase domains before measuring the obstacle-dependent diffusion coefficient. Lateral obstructed diffusion was found to be dependent on obstacle area fraction, size, and geometry. Analysis of our results using a free area diffusion model shows the possibility of unexpected long-range ordering of fluid-phase lipids around the gel-phase obstacles. This lipid ordering has implications for lipid-mediated protein interactions in cellular membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.