ArticleBiophysical journal2002
Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 119 citations in OpenAlex.
- Quantifying and Utilizing Electroosmotic Flow for Mechanical Measurements with the Scanning Ion Conductance Microscope.Analytical chemistry · 2025Article
- Operando Scanning Electrochemical Probe Microscopy during Electrocatalysis.Chemical reviews · 2023Review
- Sensing Cells-Peptide Hydrogel Interaction In Situ via Scanning Ion Conductance Microscopy.Cells · 2022Article
- Electrophysiology and Fluorescence Spectroscopy Approaches for Evaluating Gamete and Embryo Functionality in Animals and Humans.Biomolecules · 2022Review
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Scanning ion conductance microscopy of live human glomerulus.Journal of cellular and molecular medicine · 2021Article
- Scalable fabrication of sub-10 nm polymer nanopores for DNA analysis.Microsystems & nanoengineering · 2019Article
- Review
- Angular Approach Scanning Ion Conductance Microscopy.Biophysical journal · 2016Article
- T-type Ca2+ channels in spermatogenic cells and sperm.Pflugers Archiv : European journal of physiology · 2014Review
- Article
- Super-resolution scanning patch clamp reveals clustering of functional ion channels in adult ventricular myocyte.Circulation research · 2013Article
- Imaging the cell surface and its organization down to the level of single molecules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
- Article
- Rediscovering sperm ion channels with the patch-clamp technique.Molecular human reproduction · 2011Review
- Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells.Journal of the Royal Society, Interface · 2011Review
- Nanoscale live-cell imaging using hopping probe ion conductance microscopy.Nature methods · 2009Article
- Effects of lipids on ENaC activity in cultured mouse cortical collecting duct cells.The Journal of membrane biology · 2009Article
- Method of creating a nanopore-terminated probe for single-molecule enantiomer discrimination.Analytical chemistry · 2009Article
Corrections and comments
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Authors and funding
15 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a scanning patch-clamp technique that facilitates single-channel recording from small cells and submicron cellular structures that are inaccessible by conventional methods. The scanning patch-clamp technique combines scanning ion conductance microscopy and patch-clamp recording through a single glass nanopipette probe. In this method the nanopipette is first scanned over a cell surface, using current feedback, to obtain a high-resolution topographic image. This same pipette is then used to make the patch-clamp recording. Because image information is obtained via the patch electrode it can be used to position the pipette onto a cell with nanometer precision. The utility of this technique is demonstrated by obtaining ion channel recordings from the top of epithelial microvilli and openings of cardiomyocyte T-tubules. Furthermore, for the first time we have demonstrated that it is possible to record ion channels from very small cells, such as sperm cells, under physiological conditions as well as record from cellular microstructures such as submicron neuronal processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.