ArticleBiophysical journal2002
The synaptic vesicle protein synaptophysin: purification and characterization of its channel activity.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 42 citations in OpenAlex.
- Exploration of the Shared Molecular Mechanisms between COVID-19 and Neurodegenerative Diseases through Bioinformatic Analysis.International journal of molecular sciences · 2023Article
- Depletion of acetate-producing bacteria from the gut microbiota facilitates cognitive impairment through the gut-brain neural mechanism in diabetic mice.Microbiome · 2021Article
- The Phosphoprotein Synapsin Ia Regulates the Kinetics of Dense-Core Vesicle Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Room for Two: The Synaptophysin/Synaptobrevin Complex.Frontiers in synaptic neuroscience · 2021Article
- Thrombospondin-1 secreted by human umbilical cord blood-derived mesenchymal stem cells rescues neurons from synaptic dysfunction in Alzheimer's disease model.Scientific reports · 2018Article
- Altered expression and localization of synaptophysin in developing cerebellar cortex of neonatal rats due to maternal diabetes mellitus.Metabolic brain disease · 2016Article
- Architecture of the Synaptophysin/Synaptobrevin Complex: Structural Evidence for an Entropic Clustering Function at the Synapse.Scientific reports · 2015Article
- Functional ryanodine receptors in the membranes of neurohypophysial secretory granules.The Journal of general physiology · 2014Article
- The Sybtraps: control of synaptobrevin traffic by synaptophysin, α-synuclein and AP-180.Traffic (Copenhagen, Denmark) · 2014Review
- Abnormal synaptic vesicle biogenesis in Drosophila synaptogyrin mutants.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- The association of dynamin with synaptophysin regulates quantal size and duration of exocytotic events in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Structure of synaptophysin: a hexameric MARVEL-domain channel protein.Structure (London, England : 1993) · 2007Article
- Tactile stimulation-induced rapid elevation of the synaptophysin mRNA expression level in rat somatosensory cortex.Molecular and cellular biochemistry · 2006Article
- Synaptogenesis in the Cerebellum of Offspring Born to Diabetic Mothers.Journal of pediatric neurosciencesReview
- Clinical neuropathology practice guide 5-2013: markers of neuronal maturation.Clinical neuropathologyReview
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The synaptic vesicle protein synaptophysin was solubilized from rat brain synaptosomes with a relatively low concentration of Triton X-100 (0.2%) and was highly purified (above 95%) using a rapid single chromatography step on hydroxyapatite/celite resin. Purified synaptophysin was reconstituted into a planar lipid bilayer and the channel activity of synaptophysin was characterized. In asymmetric KCl solutions (cis 300 mM/trans 100 mM), synaptophysin formed a fast-fluctuating channel with a conductance of 414 +/- 13 pS at +60 mV. The open probability of synaptophysin channels was decreased upon depolarization, and channels were found to be cation-selective. Synaptophysin channels showed higher selectivity for K(+) over Cl(-) (P(K(+))/P(Cl(-)) > 8) and preferred K(+) over Li(+), Na(+), Rb(+), Cs(+), or choline(+). The synaptophysin channel is impermeable to Ca(2+), which has no effect on its channel activity. This study is the second demonstration of purified synaptophysin channel activity, but the first biophysical characterization of its channel properties. The availability of large amounts of purified synaptophysin and of its characteristic channel properties might help to establish the role of synaptophysin in synaptic transmission.
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