ArticleBiophysical journal2002
Micromechanical mapping of live cells by multiple-particle-tracking microrheology.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers.
What it found
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Who cites it
132 citing papers in PubMed, 404 citations in OpenAlex.
- Mechanobiology of the tumor microenvironment: a review of therapeutic interactions and in vitro elasticity measurement techniques.Journal of biomedical science · 2026Review
- Strategies to control cellular spatial organization in microphysiological systems.Microsystems & nanoengineering · 2026Review
- Microrheology: From Video Microscopy to Optical Tweezers.Micromachines · 2025Review
- Mechanical deformation and death of circulating tumor cells in the bloodstream.Cancer metastasis reviews · 2024Review
- Mathematical Models for Ultrasound Elastography: Recent Advances to Improve Accuracy and Clinical Utility.Bioengineering (Basel, Switzerland) · 2024Review
- Article
- Viscoelasticity of diverse biological samples quantified by Acoustic Force Microrheology (AFMR).Communications biology · 2024Article
- Multiscale elasticity mapping of biological samples in 3D at optical resolution.Acta biomaterialia · 2024Article
- From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.Frontiers in cell and developmental biology · 2024Review
- How it feels in a cell.Trends in cell biology · 2023Review
- Studying fluctuating trajectories of optically confined passive tracers inside cells provides familiar active forces.Biomedical optics express · 2023Article
- Three-dimensional single particle tracking using 4π self-interference of temporally phase-shifted fluorescence.Light, science & applications · 2023Article
- Strain-dependent elastography of cancer cells reveals heterogeneity and stiffening due to attachment.Journal of biomechanics · 2023Article
- Microfluidic techniques for mechanical measurements of biological samples.Biophysics reviews · 2023Review
- Size- and position-dependent cytoplasm viscoelasticity through hydrodynamic interactions with the cell surface.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Quantifying stiffness and forces of tumor colonies and embryos using a magnetic microrobot.Science robotics · 2023Article
- Article
- Contribution of cytoplasm viscoelastic properties to mitotic spindle positioning.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Passive and Active Microrheology for Biomedical Systems.Frontiers in bioengineering and biotechnology · 2022Review
- 2D-GolgiTrack-a semi-automated tracking system to quantify morphological changes and dynamics of the Golgi apparatus and Golgi-derived membrane tubules.Medical & biological engineering & computing · 2022Article
72 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This paper introduces the method of live-cell multiple-particle-tracking microrheology (MPTM), which quantifies the local mechanical properties of living cells by monitoring the Brownian motion of individual microinjected fluorescent particles. Particle tracking of carboxylated microspheres imbedded in the cytoplasm produce spatial distributions of cytoplasmic compliances and frequency-dependent viscoelastic moduli. Swiss 3T3 fibroblasts are found to behave like a stiff elastic material when subjected to high rates of deformations and like a soft liquid at low rates of deformations. By analyzing the relative contributions of the subcellular compliances to the mean compliance, we find that the cytoplasm is much more mechanically heterogeneous than reconstituted actin filament networks. Carboxylated microspheres embedded in cytoplasm through endocytosis and amine-modified polystyrene microspheres, which are microinjected or endocytosed, often show directed motion and strong nonspecific interactions with cytoplasmic proteins, which prevents computation of local moduli from the microsphere displacements. Using MPTM, we investigate the mechanical function of alpha-actinin in non-muscle cells: alpha-actinin-microinjected cells are stiffer and yet mechanically more heterogeneous than control cells, in agreement with models of reconstituted cross-linked actin filament networks. MPTM is a new type of functional microscopy that can test the local, rate-dependent mechanical and ultrastructural properties of living cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.