ArticleBiophysical journal2002
The motion of a single molecule, the lambda-receptor, in the bacterial outer membrane.
Article in Biophysical journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 61 citations in OpenAlex.
- Article
- Similarly slow diffusion of BAM and SecYEG complexes in live E. coli cells observed with 3D spt-PALM.Biophysical journal · 2023Article
- Space and time on the membrane: modelling Type VI secretion system dynamics as a state-dependent random walk.Royal Society open science · 2023Article
- Protection of bacteriophage-sensitiveProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The Fluidity of the Bacterial Outer Membrane Is Species Specific: Bacterial Lifestyles and the Emergence of a Fluid Outer Membrane.BioEssays : news and reviews in molecular, cellular and developmental biology · 2020Article
- Role of the lipid bilayer in outer membrane protein folding in Gram-negative bacteria.The Journal of biological chemistry · 2020Review
- Complex Diffusion in Bacteria.Advances in experimental medicine and biology · 2020Review
- Errors in Energy Landscapes Measured with Particle Tracking.Biophysical journal · 2018Article
- How Important Is Protein Diffusion in Prokaryotes?Frontiers in molecular biosciences · 2018Review
- Super-Resolution Imaging of Protein Secretion Systems and the Cell Surface of Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2017Review
- Article
- Protein-protein interactions and the spatiotemporal dynamics of bacterial outer membrane proteins.Current opinion in structural biology · 2015Review
- Article
- Dynamics of the serine chemoreceptor in the Escherichia coli inner membrane: a high-speed single-molecule tracking study.Biophysical journal · 2014Article
- Receptor displacement in the cell membrane by hydrodynamic force amplification through nanoparticles.Biophysical journal · 2013Article
- Absence of long-range diffusion of OmpA in E. coli is not caused by its peptidoglycan binding domain.BMC microbiology · 2013Article
- Single-molecule imaging in live bacteria cells.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Deciphering protein dynamics of the siderophore pyoverdine pathway in Pseudomonas aeruginosa.PloS one · 2013Article
- Bayesian decision tree for the classification of the mode of motion in single-molecule trajectories.PloS one · 2013Article
- Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using optical tweezers and single particle tracking, we have revealed the motion of a single protein, the lambda-receptor, in the outer membrane of living Escherichia coli bacteria. We genetically modified the lambda-receptor placing a biotin on an extracellular site of the receptor in vivo. The efficiency of this in vivo biotinylation is very low, thus enabling the attachment of a streptavidin-coated bead binding specifically to a single biotinylated lambda-receptor. The bead was used as a handle for the optical tweezers and as a marker for the single particle tracking routine. We propose a model that allows extraction of the motion of the protein from measurements of the mobility of the bead-molecule complex; these results are equally applicable to analyze bead-protein complexes in other membrane systems. Within a domain of radius approximately 25 nm, the receptor diffuses with a diffusion constant of (1.5 +/- 1.0) x 10(-9) cm(2)/s and sits in a harmonic potential as if it were tethered by an elastic spring of spring constant of ~1.0 x 10(-2) pN/nm to the bacterial membrane. The purpose of the protein motion might be to facilitate transport of maltodextrins through the outer bacterial membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.