ArticleNucleic acids research2002
Novel retroviral vectors to facilitate expression screens in mammalian cells.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 37 citations in OpenAlex.
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- Cells determine cell density using a small protein bound to a unique tissue-specific phospholipid.PeerJ · 2013Article
- FAM83A confers EGFR-TKI resistance in breast cancer cells and in mice.The Journal of clinical investigation · 2012Article
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- Small-molecule hydrophobic tagging-induced degradation of HaloTag fusion proteins.Nature chemical biology · 2011Article
- Making sense of cancer genomic data.Genes & development · 2011Review
- Stage-specific signaling through TGFβ family members and WNT regulates patterning and pancreatic specification of human pluripotent stem cells.Development (Cambridge, England) · 2011Article
- Cell entry of enveloped viruses.Advances in genetics · 2011Review
- Transforming JAK1 mutations exhibit differential signalling, FERM domain requirements and growth responses to interferon-γ.The Biochemical journal · 2010Article
- Epigenetic memory in induced pluripotent stem cells.Nature · 2010Article
- A herpes simplex virus vector system for expression of complex cellular cDNA libraries.Journal of virology · 2010Article
- A cell motility screen reveals role for MARCKS-related protein in adherens junction formation and tumorigenesis.PloS one · 2009Article
- A JAK2 interdomain linker relays Epo receptor engagement signals to kinase activation.The Journal of biological chemistry · 2009Article
- Integrative genomic approaches to understanding cancer.Biochimica et biophysica acta · 2009Review
- Anchorage-independent growth of pocket protein-deficient murine fibroblasts requires bypass of G2 arrest and can be accomplished by expression of TBX2.Molecular and cellular biology · 2008Article
- In vitro genetic screen identifies a cooperative role for LPA signaling and c-Myc in cell transformation.Oncogene · 2008Article
- Transformation of hematopoietic cells and activation of JAK2-V617F by IL-27R, a component of a heterodimeric type I cytokine receptor.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
As tools for functional genomics, expression profiling and proteomics provide correlative data, while expression cloning screens can link genes directly to biological function. However, technical limitations of gene transfer, expression, and recovery of candidate genes have limited wider application of genome-wide expression screens. Here we describe the pEYK retroviral vectors, which maintain high titers and robust gene expression while addressing the major bottleneck of expression cloning--efficient candidate gene recovery. By exploiting schemes for enhanced PCR rescue or strategies for direct isolation of proviral DNA as plasmids in bacterial hosts, the pEYK vectors facilitate cDNA isolation from selected cells and enable rapid iteration of screens and genetic reversion analyses to validate gene candidates. These vectors have proven useful to identify genes linked to cell proliferation, senescence and apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.