ArticleNucleic acids research2002
Studies on the minimal lengths required for DNA primers to be extended by the Tetrahymena telomerase: implications for primer positioning by the enzyme.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 23 citations in OpenAlex.
- DNA-binding determinants and cellular thresholds for human telomerase repeat addition processivity.The EMBO journal · 2017Article
- Telomerase Mechanism of Telomere Synthesis.Annual review of biochemistry · 2017Review
- Article
- Tracing the path of DNA substrates in active Tetrahymena telomerase holoenzyme complexes: mapping of DNA contact sites in the RNA subunit.Nucleic acids research · 2012Article
- InTERTpreting telomerase structure and function.Nucleic acids research · 2010Review
- Multiple mechanisms for elongation processivity within the reconstituted tetrahymena telomerase holoenzyme.The Journal of biological chemistry · 2010Article
- The telomerase-specific T motif is a restrictive determinant of repetitive reverse transcription by human telomerase.Molecular and cellular biology · 2010Article
- Multiple DNA-binding sites in Tetrahymena telomerase.Nucleic acids research · 2008Article
- High-resolution physical and functional mapping of the template adjacent DNA binding site in catalytically active telomerase.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Characterization of physical and functional anchor site interactions in human telomerase.Molecular and cellular biology · 2007Article
- The biogenesis and regulation of telomerase holoenzymes.Nature reviews. Molecular cell biology · 2006Review
- A physical and functional constituent of telomerase anchor site.The Journal of biological chemistry · 2005Article
- An anchor site-type defect in human telomerase that disrupts telomere length maintenance and cellular immortalization.Molecular biology of the cell · 2005Article
- Human telomerase catalyzes nucleolytic primer cleavage.Nucleic acids research · 2004Article
- Yeast telomerase is capable of limited repeat addition processivity.Nucleic acids research · 2004Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Telomerase is a specialized reverse transcriptase that contains an integral RNA subunit including a short template sequence. It extends telomeric 3' overhangs and chromosome breakpoints by catalyzing reiterative copying of this internal template into single-stranded telomeric DNA repeats. Here we report for the first time that in vitro the ciliate Tetrahymena telomerase can efficiently extend very short single-stranded DNA primers (<6 nt). These data indicate that interactions with nucleotides further upstream are not essential for elongation of longer primers. We also report that the minimal lengths required for primers to be extended by the telomerase depend on the positions along the template at which the primers are initially aligned. At a primer concentration of 2.5 micro M, primers aligned in the beginning, middle and next to the end of the template, respectively, must consist of at least 4, 5 and 6 nt to be extended by the telomerase. At a primer concentration of 50 micro M, the corresponding minimal lengths are 3, 4 and 5 nt. The systematic variation of the minimal required primer lengths supports the presence of a site within the telomerase ribonucleoprotein complex that mediates specific positioning of 3' termini of telomeric and non-telomeric DNA in the beginning of the template during telomere synthesis.
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