ArticleNucleic acids research2002
Identification of cellular mRNA targets for RNA-binding protein Sam68.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 68 citations in OpenAlex.
- RNA-binding proteins in bone pathophysiology.Frontiers in cell and developmental biology · 2024Review
- Article
- Article
- Intrinsic Regulatory Role of RNA Structural Arrangement in Alternative Splicing Control.International journal of molecular sciences · 2020Review
- β-Actin mRNA interactome mapping by proximity biotinylation.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- SAM68 interaction with U1A modulates U1 snRNP recruitment and regulates mTor pre-mRNA splicing.Nucleic acids research · 2019Article
- Unraveling the Pathways to Neuronal Homeostasis and Disease: Mechanistic Insights into the Role of RNA-Binding Proteins and Associated Factors.International journal of molecular sciences · 2018Review
- Imaging mRNA and protein interactions within neurons.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Structural basis of RNA recognition and dimerization by the STAR proteins T-STAR and Sam68.Nature communications · 2016Article
- Proteostasis and RNA Binding Proteins in Synaptic Plasticity and in the Pathogenesis of Neuropsychiatric Disorders.Neural plasticity · 2016Review
- Analysis of the interaction between host factor Sam68 and viral elements during foot-and-mouth disease virus infections.Virology journal · 2015Article
- Impacts of Alternative Splicing Events on the Differentiation of Adipocytes.International journal of molecular sciences · 2015Review
- Homoiterons and expansion in ribosomal RNAs.FEBS open bio · 2015Article
- SAM68: Signal Transduction and RNA Metabolism in Human Cancer.BioMed research international · 2015Review
- Respiratory virus modulation of host nucleocytoplasmic transport; target for therapeutic intervention?Frontiers in microbiology · 2015Review
- Evolutionary Dynamics of GLD-1-mRNA complexes in Caenorhabditis nematodes.Genome biology and evolution · 2014Article
- Screening protein--single stranded RNA complexes by NMR spectroscopy for structure determination.Methods (San Diego, Calif.) · 2014Article
- Role of Sam68 in post-transcriptional gene regulation.International journal of molecular sciences · 2013Review
- RNA-binding protein Sam68 controls synapse number and local β-actin mRNA metabolism in dendrites.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Neurodegenerative diseases: quantitative predictions of protein-RNA interactions.RNA (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sam68 (Src-associated in mitosis, 68 kDa), a nuclear RNA-binding protein, has been postulated to play a role in cell-growth control as a modulator of signal transduction and activation of RNA metabolism. Although Sam68 was demonstrated to bind to the UAAA sequences in synthetic oligoribonucleotides and poly(U) homopolymers in vitro, the legitimate cellular mRNA target remained unclear. By using the differential display and cDNA-representational difference analysis techniques, followed by reverse transcription polymerase chain reaction of RNAs co-immunoprecipitated with Sam68 from a HeLa cell lysate, we identified 10 mRNA species that bind in vivo to Sam68 in an RNA-binding domain-dependent manner. Among them, the mRNA species for hnRNP A2/B1 and beta-actin were found to bind prominently in vivo as well as in vitro, suggesting the possible involvement of Sam68 in the post- transcriptional regulation of these genes. Mapping of the Sam68-binding sequence revealed that Sam68 associates with these mRNAs through different nucleotide motifs, UAAA for hnRNP A2/B1 mRNA and UUUUUU for beta-actin mRNA, and that both binding sequences must reside in a loop structure for recognition by Sam68. The results indicated that Sam68 recognizes both the UAAA motif and poly(U) sequences in vivo for binding to cellular target mRNAs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.