ArticleProceedings of the National Academy of Sciences of the United States of America2003
RNA polymerase II-dependent transcription in trypanosomes is associated with a SNAP complex-like transcription factor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed.
- TBP and SNAP50 transcription factors bind specifically to the Pr77 promoter sequence from trypanosomatid non-LTR retrotransposons.Parasites & vectors · 2021Article
- Transcription Factor Analysis in Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Transcriptional regulation of snRNAs and its significance for plant development.Journal of plant research · 2017Review
- The Myb domain of the largest subunit of SNAPc adopts different architectural configurations on U1 and U6 snRNA gene promoter sequences.Nucleic acids research · 2014Article
- Architectural arrangement of the small nuclear RNA (snRNA)-activating protein complex 190 subunit (SNAP190) on U1 snRNA gene promoter DNA.The Journal of biological chemistry · 2012Article
- Mutation of zebrafish Snapc4 is associated with loss of the intrahepatic biliary network.Developmental biology · 2012Article
- Development of an efficient in vitro transcription system for bloodstream form Trypanosoma brucei reveals life cycle-independent functionality of class I transcription factor A.Molecular and biochemical parasitology · 2012Article
- A TFIIH-associated mediator head is a basal factor of small nuclear spliced leader RNA gene transcription in early-diverged trypanosomes.Molecular and cellular biology · 2010Article
- Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.Molecular and cellular biology · 2010Article
- Gene expression in trypanosomatid parasites.Journal of biomedicine & biotechnology · 2010Review
- Regulation of gene expression in protozoa parasites.Journal of biomedicine & biotechnology · 2010Review
- A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.The Journal of biological chemistry · 2009Article
- Structure of the C-terminal domain of transcription factor IIB from Trypanosoma brucei.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Transcriptional regulation of human small nuclear RNA genes.Biochimica et biophysica acta · 2008Review
- Differential requirement for the function of SRD2, an snRNA transcription activator, in various stages of plant development.Plant molecular biology · 2008Article
- Spliced leader RNA gene transcription in Trypanosoma brucei requires transcription factor TFIIH.Eukaryotic cell · 2007Article
- Insect small nuclear RNA gene promoters evolve rapidly yet retain conserved features involved in determining promoter activity and RNA polymerase specificity.Nucleic acids research · 2007Article
- Trypanosomal TBP functions with the multisubunit transcription factor tSNAP to direct spliced-leader RNA gene expression.Molecular and cellular biology · 2005Article
- Characterization of a multisubunit transcription factor complex essential for spliced-leader RNA gene transcription in Trypanosoma brucei.Molecular and cellular biology · 2005Article
- Actively transcribing RNA polymerase II concentrates on spliced leader genes in the nucleus of Trypanosoma cruzi.Eukaryotic cell · 2005Article
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2 authors.
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Abstract
Spliced leader RNA transcription is essential for cell viability in trypanosomes. The SL RNA genes are expressed from the only defined RNA polymerase II-dependent promoter identified to date in the trypanosome genome. The SL RNA gene promoter has been shown by in vitro and in vivo analyses to have a tripartite architecture. The upstream most cis-acting element, called PBP-1E, is located between 70 and 60 bp upstream from the transcription start site. This essential element functions along with two downstream elements to direct efficient and proper initiation of transcription. Electrophoretic mobility-shift studies detected a 122-kDa protein, called PBP-1, which interacts with PBP-1E. This protein is the first sequence-specific, double-stranded DNA-binding protein isolated in trypanosomes. Three polypeptides copurify with PBP-1 activity, suggesting that PBP-1 is composed of 57-, 46-, and 36-kDa subunits. We have cloned the genes that encode the 57- and 46-kDa subunits. The 46-kDa protein is a previously uncharacterized protein and may be unique to trypanosomes. Its predicted tertiary structure suggests it binds DNA as part of a complex. The 57-kDa subunit is orthologous to the human small nuclear RNA-activating protein (SNAP)50, which is an essential subunit of the SNAP complex (SNAPc). In human cells, SNAPc binds to the proximal sequence element in both RNA polymerase II- and III-dependent small nuclear RNA gene promoters. These findings identify a surprising link in the transcriptional machinery across a large evolutionary distance in the regulation of small nuclear RNA genes in eukaryotes.
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