ArticleProceedings of the National Academy of Sciences of the United States of America2002
Dynamics of microtubule asters in microfabricated chambers: the role of catastrophes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 83 citations in OpenAlex.
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- Reliable and robust control of nucleus centering is contingent on nonequilibrium force patterns.iScience · 2023Article
- Actin network architecture can ensure robust centering or sensitive decentering of the centrosome.The EMBO journal · 2022Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Prophase-Specific Perinuclear Actin Coordinates Centrosome Separation and Positioning to Ensure Accurate Chromosome Segregation.Cell reports · 2020Article
- Physical Forces Determining the Persistency and Centering Precision of Microtubule Asters.Nature physics · 2018Article
- Physical Limits on the Precision of Mitotic Spindle Positioning by Microtubule Pushing forces: Mechanics of mitotic spindle positioning.BioEssays : news and reviews in molecular, cellular and developmental biology · 2017Review
- Polarity Reversal by Centrosome Repositioning Primes Cell Scattering during Epithelial-to-Mesenchymal Transition.Developmental cell · 2017Article
- Probing the biology of cell boundary conditions through confinement of Xenopus cell-free cytoplasmic extracts.Genesis (New York, N.Y. : 2000) · 2017Review
- A Motor-Gradient and Clustering Model of the Centripetal Motility of MTOCs in Meiosis I of Mouse Oocytes.PLoS computational biology · 2016Article
- Centrosome centering and decentering by microtubule network rearrangement.Molecular biology of the cell · 2016Article
- Understanding force-generating microtubule systems through in vitro reconstitution.Cell adhesion & migration · 2016Review
- Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets.Journal of visualized experiments : JoVE · 2016Article
- Buckling of Microtubules on a 2D Elastic Medium.Scientific reports · 2015Article
- Biological Scaling Problems and Solutions in Amphibians.Cold Spring Harbor perspectives in biology · 2015Review
- Cell size modulates oscillation, positioning and length of mitotic spindles.Scientific reports · 2015Article
- Experiments inside a box lead to out-of-the-box ideas on cellular organization.Systems and synthetic biology · 2014Article
- Motor-mediated cortical versus astral microtubule organization in lipid-monolayered droplets.The Journal of biological chemistry · 2014Article
- What determines cell size?BMC biology · 2012Article
- End-on microtubule-dynein interactions and pulling-based positioning of microtubule organizing centers.Cell cycle (Georgetown, Tex.) · 2012Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent in vivo as well as in vitro experiments have indicated that microtubule pushing alone is sufficient to position a microtubule-organizing center within a cell. Here, we investigate the effect of catastrophes on the dynamics of microtubule asters within microfabricated chambers that mimic the confining geometry of living cells. The use of a glass bead as the microtubule-organizing center allows us to manipulate the aster by using optical tweezers. In the case in which microtubules preexist, we show that because of microtubule buckling, repositioning almost never occurs after relocation with the optical tweezers, although initial microtubule growth always leads the aster to the geometrical center of the chamber. When a catastrophe promoter is added, we find instead that the aster is able to efficiently explore the chamber geometry even after being relocated with the optical tweezers. As predicted by theoretical calculations, the results of our in vitro experiments clearly demonstrate the need for catastrophes for proper positioning in a confining geometry. These findings correlate with recent observations of nuclear positioning in fission yeast cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.