ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2002
Timing and efficacy of Ca2+ channel activation in hippocampal mossy fiber boutons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers.
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Who cites it
99 citing papers in PubMed.
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- The plasticity of pyramidal neurons in the behaving brain.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Human iPSC-Derived Neurons with Reliable Synapses and Large Presynaptic Action Potentials.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Enhanced excitability but mature action potential waveforms at mossy fiber terminals of young, adult-born hippocampal neurons in mice.Communications biology · 2023Article
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- The computational power of the human brain.Frontiers in cellular neuroscience · 2023Review
- Upregulated CaCells · 2022Article
- Multiscale modeling of presynaptic dynamics from molecular to mesoscale.PLoS computational biology · 2022Article
- Modeling analysis of subthreshold voltage signaling along hippocampal mossy fiber axons.Frontiers in cellular neuroscience · 2022Article
- Using ephaptic coupling to estimate the synaptic cleft resistivity of the calyx of Held synapse.PLoS computational biology · 2021Article
- Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses.Nature protocols · 2021Article
- Functional Microstructure of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Presynaptic endoplasmic reticulum regulates short-term plasticity in hippocampal synapses.Communications biology · 2021Article
- Markov Chain Abstractions of Electrochemical Reaction-Diffusion in Synaptic Transmission for Neuromorphic Computing.Frontiers in neuroscience · 2021Article
- Synaptic Organization of the Human Temporal Lobe Neocortex as Revealed by High-Resolution Transmission, Focused Ion Beam Scanning, and Electron Microscopic Tomography.International journal of molecular sciences · 2020Article
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- Axonal NaScience advances · 2020Article
- Presynaptic calcium channels: specialized control of synaptic neurotransmitter release.Nature reviews. Neuroscience · 2020Review
39 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The presynaptic Ca2+ signal is a key determinant of transmitter release at chemical synapses. In cortical synaptic terminals, however, little is known about the kinetic properties of the presynaptic Ca2+ channels. To investigate the timing and magnitude of the presynaptic Ca2+ inflow, we performed whole-cell patch-clamp recordings from mossy fiber boutons (MFBs) in rat hippocampus. MFBs showed large high-voltage-activated Ca(2+) currents, with a maximal amplitude of approximately 100 pA at a membrane potential of 0 mV. Both activation and deactivation were fast, with time constants in the submillisecond range at a temperature of approximately 23 degrees C. An MFB action potential (AP) applied as a voltage-clamp command evoked a transient Ca2+ current with an average amplitude of approximately 170 pA and a half-duration of 580 microsec. A prepulse to +40 mV had only minimal effects on the AP-evoked Ca2+ current, indicating that presynaptic APs open the voltage-gated Ca2+ channels very effectively. On the basis of the experimental data, we developed a kinetic model with four closed states and one open state, linked by voltage-dependent rate constants. Simulations of the Ca2+ current could reproduce the experimental data, including the large amplitude and rapid time course of the current evoked by MFB APs. Furthermore, the simulations indicate that the shape of the presynaptic AP and the gating kinetics of the Ca2+ channels are tuned to produce a maximal Ca2+ influx during a minimal period of time. The precise timing and high efficacy of Ca2+ channel activation at this cortical glutamatergic synapse may be important for synchronous transmitter release and temporal information processing.
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12486151PMC6758411What OpenQuestion holds
Registered trials
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