ArticleThe EMBO journal2002
An inserted region of leucyl-tRNA synthetase plays a critical role in group I intron splicing.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 43 citations in OpenAlex.
- Maturase K forms a plastidial splicing complex with a neofunctionalized branching enzyme.Nature communications · 2026Article
- Compendium of Methods to Uncover RNA-Protein Interactions In Vivo.Methods and protocols · 2021Review
- The Ribosome as a Missing Link in Prebiotic Evolution III: Over-Representation of tRNA- and rRNA-Like Sequences and Plieofunctionality of Ribosome-Related Molecules Argues for the Evolution of Primitive Genomes from Ribosomal RNA Modules.International journal of molecular sciences · 2019Article
- Human mitochondrial leucyl tRNA synthetase can suppress non cognate pathogenic mt-tRNA mutations.EMBO molecular medicine · 2014Article
- Emergence and evolution.Topics in current chemistry · 2014Review
- Yeast mitochondrial leucyl-tRNA synthetase CP1 domain has functionally diverged to accommodate RNA splicing at expense of hydrolytic editing.The Journal of biological chemistry · 2012Article
- Orthogonal use of a human tRNA synthetase active site to achieve multifunctionality.Nature structural & molecular biology · 2010Article
- Leucyl-tRNA synthetase-dependent and -independent activation of a group I intron.The Journal of biological chemistry · 2009Article
- Evolutionary basis for the coupled-domain motions in Thermus thermophilus leucyl-tRNA synthetase.The Journal of biological chemistry · 2009Article
- CP1-dependent partitioning of pretransfer and posttransfer editing in leucyl-tRNA synthetase.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.FEMS microbiology reviews · 2008Review
- A shared RNA-binding site in the Pet54 protein is required for translational activation and group I intron splicing in yeast mitochondria.Nucleic acids research · 2008Article
- Implication of leucyl-tRNA synthetase 1 (LARS1) over-expression in growth and migration of lung cancer cells detected by siRNA targeted knock-down analysis.Experimental & molecular medicine · 2008Article
- A C-terminal fragment of an intron-encoded maturase is sufficient for promoting group I intron splicing.RNA (New York, N.Y.) · 2005Article
- A domain for editing by an archaebacterial tRNA synthetase.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- tRNA synthetase: tRNA aminoacylation and beyond.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
Yeast mitochondrial leucyl-tRNA synthetase (LeuRS) binds to the bI4 intron and collaborates with the bI4 maturase to aid excision of the group I intron. Deletion analysis isolated the inserted LeuRS CP1 domain as a critical factor in the protein's splicing activity. Protein fragments comprised of just the LeuRS CP1 region rescued complementation of a yeast strain that expressed a splicing-defective LeuRS. Three-hybrid analysis determined that these CP1-containing LeuRS fragments, ranging from 214 to 375 amino acids, bound to the bI4 intron. In each case, interactions with only the LeuRS protein fragment specifically stimulated bI4 intron splicing activity. Substitution of a homologous CP1 domain from isoleucyl-tRNA synthetase or mutation within the LeuRS CP1 region of the smallest protein fragment abolished RNA binding and splicing activity. The CP1 domain is best known for its amino acid editing activity. However, these results suggest that elements within the LeuRS CP1 domain also play a novel role, independent of the full-length tRNA synthetase, in binding the bI4 group I intron and facilitating its self-splicing activity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.