ArticleThe EMBO journal2002
PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 68 citations in OpenAlex.
- Subcellular compartmentalization dependent dichotomy of p21 in cancer.Molecular biology reports · 2026Review
- MiR-3202-DTL signaling axis impedes NSCLC malignancy via regulating the ubiquitination-proteasome degradation of p21.Molecular and cellular biochemistry · 2025Article
- PKC signal amplification suppresses non-small cell lung cancer growth by promoting p21 expression and phosphorylation.Heliyon · 2022Article
- PI3K Promotes Basal Cell Carcinoma Growth Through Kinase-Induced p21 Degradation.Frontiers in oncology · 2021Article
- The Role of the Cyclin Dependent Kinase Inhibitor p21Cancers · 2019Review
- p21cip1/waf1 Coordinate Autophagy, Proliferation and Apoptosis in Response to Metabolic Stress.Cancers · 2019Review
- Review
- PKCι depletion initiates mitotic slippage-induced senescence in glioblastoma.Cell cycle (Georgetown, Tex.) · 2015Article
- Binding of Kif23-iso1/CHO1 to 14-3-3 is regulated by sequential phosphorylations at two LATS kinase consensus sites.PloS one · 2015Article
- aPKC phosphorylates p27Xic1, providing a mechanistic link between apicobasal polarity and cell-cycle control.Developmental cell · 2014Article
- Bimodal regulation of p21(waf1) protein as function of DNA damage levels.Cell cycle (Georgetown, Tex.) · 2014Article
- The p90 ribosomal S6 kinase (RSK) inhibitor BI-D1870 prevents gamma irradiation-induced apoptosis and mediates senescence via RSK- and p53-independent accumulation of p21WAF1/CIP1.Cell death & disease · 2013Article
- Expression of proviral integration site for Moloney murine leukemia virus 1 (Pim-1) is post-transcriptionally regulated by tristetraprolin in cancer cells.The Journal of biological chemistry · 2012Article
- Repression of cancer cell senescence by PKCι.Oncogene · 2012Article
- Protein kinase C signaling and cell cycle regulation.Frontiers in immunology · 2012Article
- Article
- Cdk2-dependent phosphorylation of p21 regulates the role of Cdk2 in cisplatin cytotoxicity.American journal of physiology. Renal physiology · 2011Article
- Human NDR kinases control G(1)/S cell cycle transition by directly regulating p21 stability.Molecular and cellular biology · 2011Article
- Article
- Examination of the expanding pathways for the regulation of p21 expression and activity.Cellular signalling · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
p21(WAF1/CIP1) Contributes to positive and negative growth control on multiple levels. We previously mapped phosphorylation sites within the C-terminal domain of p21 that regulate proliferating cell nuclear antigen binding. In the current study, a kinase has been fractionated from mammalian cells that stoichiometrically phosphorylates p21 at the Ser146 site, and the enzyme has been identified as an insulin-responsive atypical protein kinase C (aPKC). Expression of PKCzeta or activation of the endogenous kinase by 3-phosphoinositide dependent protein kinase-1 (PDK1) decreased the half-life of p21. Conversely, dnPKCzeta or dnPDK1 increased p21 protein half-life, and a PDK1-dependent increase in the rate of p21 degradation was mediated by aPKC. Insulin stimulation gave a biphasic response with a rapid transient decrease in p21 protein levels during the initial signalling phase that was dependent on phosphatidylinositol 3- kinase, PKC and proteasome activity. Thus, aPKC provides a physiological signal for the degradation of p21. The rapid degradation of p21 protein during the signalling phase of insulin stimulation identifies a novel link between energy metabolism and a key modulator of cell cycle progression.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.