ArticleThe EMBO journal2002
Structural and functional characterization of the Pseudomonas hydroperoxide resistance protein Ohr.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 98 citations in OpenAlex.
- Linking oxidative stress defense to biofilm architecture: Ohr mediates strain-dependent persistence inVirulence · 2026Article
- Two redox-responsive LysR-type transcription factors control the oxidative stress response of Agrobacterium tumefaciens.Nucleic acids research · 2025Article
- Thiol Reductases inAntioxidants (Basel, Switzerland) · 2022Review
- Organic Hydroperoxide Resistance GeneApplied and environmental microbiology · 2021Article
- Large-scale computational discovery and analysis of virus-derived microbial nanocompartments.Nature communications · 2021Article
- The Transcriptomic Landscape ofGenes · 2020Article
- Article
- TheJournal of bacteriology · 2019Article
- Gene Regulation by Redox-Sensitive Burkholderia thailandensis OhrR and Its Role in Bacterial Killing of Caenorhabditis elegans.Infection and immunity · 2018Article
- Article
- Structural insights on the efficient catalysis of hydroperoxide reduction by Ohr: Crystallographic and molecular dynamics approaches.PloS one · 2018Article
- Cupriavidus pinatubonensis AEO106 deals with copper-induced oxidative stress before engaging in biodegradation of the herbicide 4-chloro-2-methylphenoxyacetic acid.BMC microbiology · 2017Article
- Functional and evolutionary characterization of Ohr proteins in eukaryotes reveals many active homologs among pathogenic fungi.Redox biology · 2017Article
- Ohr plays a central role in bacterial responses against fatty acid hydroperoxides and peroxynitrite.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Draft Genome Sequence of the Nitrogen-FixingFrontiers in microbiology · 2017Article
- Thiol-based switch mechanism of virulence regulator AphB modulates oxidative stress response in Vibrio cholerae.Molecular microbiology · 2016Article
- Exposure of Bacterial Biofilms to Electrical Current Leads to Cell Death Mediated in Part by Reactive Oxygen Species.PloS one · 2016Article
- Genome and Proteome Analysis of Rhodococcus erythropolis MI2: Elucidation of the 4,4´-Dithiodibutyric Acid Catabolism.PloS one · 2016Article
- Regulation of Organic Hydroperoxide Stress Response by Two OhrR Homologs in Pseudomonas aeruginosa.PloS one · 2016Article
- Inactivation of the organic hydroperoxide stress resistance regulator OhrR enhances resistance to oxidative stress and isoniazid in Mycobacterium smegmatis.Journal of bacteriology · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria have developed complex strategies to detoxify and repair damage caused by reactive oxygen species. These compounds, produced during bacterial aerobic respiration as well as by the host immune system cells as a defense mechanism against the pathogenic microorganisms, have the ability to damage nucleic acids, proteins and phospholipid membranes. Here we describe the crystal structure of Pseudomonas aeruginosa Ohr, a member of a recently discovered family of organic hydroperoxide resistance proteins. Ohr is a tightly folded homodimer, with a novel alpha/beta fold, and contains two active sites located at the monomer interface on opposite sides of the molecule. Using in vitro assays, we demonstrate that Ohr functions directly as a hydroperoxide reductase, converting both inorganic and organic hydroperoxides to less toxic metabolites. Site-directed mutagenesis confirms that the two conserved cysteines in each active site are essential for catalytic activity. We propose that the Ohr catalytic mechanism is similar to that of the structurally unrelated peroxiredoxins, directly utilizing highly reactive cysteine thiol groups to elicit hydroperoxide reduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.