ArticleJournal of the American Society for Mass Spectrometry2002
Identification and localization of the fatty acid modification in ghrelin by electron capture dissociation.
Article in Journal of the American Society for Mass Spectrometry, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Direct detection of S-palmitoylation by mass spectrometry.Analytical chemistry · 2013Article
- Transition metal ions: charge carriers that mediate the electron capture dissociation pathways of peptides.Journal of the American Society for Mass Spectrometry · 2011Article
- Effects of peptide backbone amide-to-ester bond substitution on the cleavage frequency in electron capture dissociation and collision-activated dissociation.Journal of the American Society for Mass Spectrometry · 2011Article
- Electron capture dissociation mass spectrometry of tyrosine nitrated peptides.Journal of the American Society for Mass Spectrometry · 2010Article
- The effect of phosphorylation on the electron capture dissociation of peptide ions.Journal of the American Society for Mass Spectrometry · 2008Article
- Liquid chromatography electron capture dissociation tandem mass spectrometry (LC-ECD-MS/MS) versus liquid chromatography collision-induced dissociation tandem mass spectrometry (LC-CID-MS/MS) for the identification of proteins.Journal of the American Society for Mass Spectrometry · 2007Article
- Electron capture dissociation of peptides metalated with alkaline-earth metal ions.Journal of the American Society for Mass Spectrometry · 2006Article
- Evaluation and optimization of electron capture dissociation efficiency in fourier transform ion cyclotron resonance mass spectrometry.Journal of the American Society for Mass Spectrometry · 2005Article
- Structural characterization of the GM1 ganglioside by infrared multiphoton dissociation, electron capture dissociation, and electron detachment dissociation electrospray ionization FT-ICR MS/MS.Journal of the American Society for Mass Spectrometry · 2005Article
- Characterization of an N-acylated glucagon-like peptide-1 derivative by electron capture dissociation.Journal of the American Society for Mass Spectrometry · 2005Article
- Detection and characterization of methionine oxidation in peptides by collision-induced dissociation and electron capture dissociation.Journal of the American Society for Mass Spectrometry · 2003Article
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron capture dissociation (ECD) has been demonstrated to be an effective fragmentation technique for characterizing the site and structure of the fatty acid modification in ghrelin, a 28-residue growth-hormone-releasing peptide that has an unusual ester-linked n-octanoyl (C8:0) modification at Ser-3. ECD cleaves 21 of 23 possible backbone amine bonds, with the product ions (c and z* ions) covering a greater amino acid sequence than those obtained by collisionally activated dissociation (CAD). Consistent with the ECD nonergodic mechanism, the ester-linked octanoyl group is retained on all backbone cleavage product ions, allowing for direct localization of this labile modification. In addition, ECD also induces the ester bond cleavage to cause the loss of octanoic acid from the ghrelin molecular ion; the elimination process is initiated by the capture of an electron at the protonated ester group, which is followed by the radical-site-initiated reaction known as alpha-cleavage. The chemical composition of the attached fatty acid can be directly obtained from the accurate Fourier transform ion cyclotron resonance (FTICR) mass measurement of the ester bond cleavage product ions.
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