ArticleMolecular and cellular biology2003
Activator-independent functions of the yeast mediator sin4 complex in preinitiation complex formation and transcription reinitiation.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- TRiC/CCT chaperonin governs RNA polymerase II activity in the nucleus to support RNA homeostasis.Molecular cell · 2026Article
- A mechanism of synergistic Mediator recruitment in RNA polymerase II transcription activation revealed by single-molecule fluorescence.Molecular cell · 2025Article
- The role of mediator subunit MED7 in Arabidopsis development.Frontiers in plant science · 2025Article
- Structural basis of a transcription pre-initiation complex on a divergent promoter.Molecular cell · 2023Article
- Yeast Mediator facilitates transcription initiation at most promoters via a Tail-independent mechanism.Molecular cell · 2022Article
- In vitro assembly and proteomic analysis of RNA polymerase II complexes.Methods (San Diego, Calif.) · 2019Article
- Transcriptome Analysis of FourG3 (Bethesda, Md.) · 2018Article
- Transcription regulation by the Mediator complex.Nature reviews. Molecular cell biology · 2018Review
- Toward understanding of the mechanisms of Mediator function in vivo: Focus on the preinitiation complex assembly.Transcription · 2017Review
- A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting.Nature communications · 2016Article
- Arabidopsis HOOKLESS1 Regulates Responses to Pathogens and Abscisic Acid through Interaction with MED18 and Acetylation of WRKY33 and ABI5 Chromatin.The Plant cell · 2016Article
- Nucleosome distortion as a possible mechanism of transcription activation domain function.Epigenetics & chromatin · 2016Review
- The Mediator complex: a central integrator of transcription.Nature reviews. Molecular cell biology · 2015Review
- Mediator redefines itself.Cell research · 2014Article
- Suppression of Mediator is regulated by Cdk8-dependent Grr1 turnover of the Med3 coactivator.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- The Arabidopsis mediator complex subunits MED16, MED14, and MED2 regulate mediator and RNA polymerase II recruitment to CBF-responsive cold-regulated genes.The Plant cell · 2014Article
- Investigating transcription reinitiation through in vitro approaches.Transcription · 2014Review
- Proteomic analysis demonstrates activator- and chromatin-specific recruitment to promoters.The Journal of biological chemistry · 2012Article
- Facilitated assembly of the preinitiation complex by separated tail and head/middle modules of the mediator.Journal of molecular biology · 2012Article
- Core promoter-selective function of HMGA1 and Mediator in Initiator-dependent transcription.Genes & development · 2011Article
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2 authors.
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Abstract
RNA polymerase II (Pol II) Mediator plays an essential role in both basal and activated transcription. Previously, subunits of the Sin4 Mediator complex (Sin4, Pgd1, Gal11, and Med2) have been implicated in both positive and negative transcriptional regulation. Furthermore, it was proposed that this subcomplex constitutes an activator-binding domain. A yeast nuclear-extract system was used to investigate the biochemical role of the Sin4 complex. In contrast to previous findings, we found at least two general activator-independent roles for the Sin4 complex. First, mutations in sin4 and pgd1 destabilized the Pol II-Med complex, leading to a reduced rate and extent of preinitiation complex (PIC) formation both in the presence and absence of activators. Although reduced in amount compared with the wild type, PICs that are formed lacking the Sin4 complex are stable and can initiate transcription normally. Second, mutation of pgd1 causes partial disruption of the Sin4 complex and leads to a defect in transcription reinitiation. This defect is caused by dissociation of mutant Mediator from promoters after initiation, leading to nonfunctional Scaffold complexes. These results show that function of the Sin4 complex is not essential for transcription activation in a crude in vitro system but that it plays key roles in the general transcription mechanism.
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