ArticleMolecular and cellular biology2003
An Rpb4/Rpb7-like complex in yeast RNA polymerase III contains the orthologue of mammalian CGRP-RCP.
Article in Molecular and cellular biology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence.Scientific reports · 2016Article
- A partial loss-of-function mutation in an Arabidopsis RNA polymerase III subunit leads to pleiotropic defects.Journal of experimental botany · 2016Article
- Subunit compositions of Arabidopsis RNA polymerases I and III reveal Pol I- and Pol III-specific forms of the AC40 subunit and alternative forms of the C53 subunit.Nucleic acids research · 2015Article
- Involvement of calcitonin gene-related peptide and receptor component protein in experimental autoimmune encephalomyelitis.Journal of neuroimmunology · 2014Article
- Role of CGRP-receptor component protein (RCP) in CLR/RAMP function.Current protein & peptide science · 2013Review
- Crystal structure of the C17/25 subcomplex from Schizosaccharomyces pombe RNA polymerase III.Protein science : a publication of the Protein Society · 2011Article
- RNA pol II subunit RPB7 is required for RNA pol I-mediated transcription in Trypanosoma brucei.EMBO reports · 2009Article
- Genomewide recruitment analysis of Rpb4, a subunit of polymerase II in Saccharomyces cerevisiae, reveals its involvement in transcription elongation.Eukaryotic cell · 2008Article
- Crystallization of RNA polymerase I subcomplex A14/A43 by iterative prediction, probing and removal of flexible regions.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Overexpression and purification of human calcitonin gene-related peptide-receptor component protein in Escherichia coli.Protein expression and purification · 2007Article
- Functional organization of the Rpb5 subunit shared by the three yeast RNA polymerases.Nucleic acids research · 2007Article
- RNA polymerase I-specific subunit CAST/hPAF49 has a role in the activation of transcription by upstream binding factor.Molecular and cellular biology · 2006Article
- The RNA polymerase I transcription machinery.Biochemical Society symposium · 2006Review
- Ancient origin, functional conservation and fast evolution of DNA-dependent RNA polymerase III.Nucleic acids research · 2006Article
- Domainal organization of the lower eukaryotic homologs of the yeast RNA polymerase II core subunit Rpb7 reflects functional conservation.Nucleic acids research · 2004Article
- Architecture of initiation-competent 12-subunit RNA polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Complete, 12-subunit RNA polymerase II at 4.1-A resolution: implications for the initiation of transcription.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Loss of the Rpb4/Rpb7 subcomplex in a mutant form of the Rpb6 subunit shared by RNA polymerases I, II, and III.Molecular and cellular biology · 2003Article
- The A14-A43 heterodimer subunit in yeast RNA pol I and their relationship to Rpb4-Rpb7 pol II subunits.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- TFIIB-related factors in RNA polymerase I transcription.Biochimica et biophysica actaReview
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10 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The essential C17 subunit of yeast RNA polymerase (Pol) III interacts with Brf1, a component of TFIIIB, suggesting a role for C17 in the initiation step of transcription. The protein sequence of C17 (encoded by RPC17) is conserved from yeasts to humans. However, mammalian homologues of C17 (named CGRP-RCP) are known to be involved in a signal transduction pathway related to G protein-coupled receptors, not in transcription. In the present work, we first establish that human CGRP-RCP is the genuine orthologue of C17. CGRP-RCP was found to functionally replace C17 in Deltarpc17 yeast cells; the purified mutant Pol III contained CGRP-RCP and had a decreased specific activity but initiated faithfully. Furthermore, CGRP-RCP was identified by mass spectrometry in a highly purified human Pol III preparation. These results suggest that CGRP-RCP has a dual function in mammals. Next, we demonstrate by genetic and biochemical approaches that C17 forms with C25 (encoded by RPC25) a heterodimer akin to Rpb4/Rpb7 in Pol II. C17 and C25 were found to interact genetically in suppression screens and physically in coimmunopurification and two-hybrid experiments. Sequence analysis and molecular modeling indicated that the C17/C25 heterodimer likely adopts a structure similar to that of the archaeal RpoE/RpoF counterpart of the Rpb4/Rpb7 complex. These RNA polymerase subunits appear to have evolved to meet the distinct requirements of the multiple forms of RNA polymerases.
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