ArticlePlant physiology2002
Expression and biochemical properties of a ferredoxin-dependent heme oxygenase required for phytochrome chromophore synthesis.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 163 citations in OpenAlex.
- Introduction of regioselective bacterial heme oxygenases into Arabidopsis hy1-1 supports the retrograde heme signaling hypothesis.Plant physiology · 2026Article
- Client distribution between Chlamydomonas FDX1 and FDX2 in carbon, nitrogen and sulfur assimilation.bioRxiv : the preprint server for biology · 2026Article
- Systems metabolic engineering ofFrontiers in plant science · 2025Article
- Regulatory effects of Hemin on prevention and rescue of salt stress in rapeseed (Brassica napus L.) seedlings.BMC plant biology · 2023Article
- Salt Stress-Induced Modulation of Porphyrin Biosynthesis, Photoprotection, and Antioxidant Properties in Rice Plants (Antioxidants (Basel, Switzerland) · 2023Article
- Bilirubin is produced nonenzymatically in plants to maintain chloroplast redox status.Science advances · 2023Article
- Exogenous Hemin alleviates NaCl stress by promoting photosynthesis and carbon metabolism in rice seedlings.Scientific reports · 2023Article
- Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase-1.FEBS open bio · 2022Article
- Carbon monoxide promotes stomatal initiation by regulating the expression of twoFrontiers in plant science · 2022Article
- Is there a role for tau glutathione transferases in tetrapyrrole metabolism and retrograde signalling in plants?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Roles and maturation of iron-sulfur proteins in plastids.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Review
- Heme oxygenase up-regulation under ultraviolet-B radiation is not epigenetically restricted and involves specific stress-related transcriptions factors.Redox biology · 2017Article
- Osmopriming-induced salt tolerance during seed germination of alfalfa most likely mediates through HProtoplasma · 2017Article
- Heme oxygenase 1 defects lead to reduced chlorophyll in Brassica napus.Plant molecular biology · 2017Article
- Effects of Light-Emitting Diode Irradiation on Growth Characteristics and Regulation of Porphyrin Biosynthesis in Rice Seedlings.International journal of molecular sciences · 2017Article
- The iron-sulfur cluster biosynthesis protein SUFB is required for chlorophyll synthesis, but not phytochrome signaling.The Plant journal : for cell and molecular biology · 2017Article
- Carbon Monoxide as a Signaling Molecule in Plants.Frontiers in plant science · 2016Review
- Comparative Transcriptome Analysis Reveals Effects of Exogenous Hematin on Anthocyanin Biosynthesis during Strawberry Fruit Ripening.International journal of genomics · 2016Article
- Identification of Crowding Stress Tolerance Co-Expression Networks Involved in Sweet Corn Yield.PloS one · 2016Article
- Differential Antioxidant Responses and Perturbed Porphyrin Biosynthesis after Exposure to Oxyfluorfen and Methyl Viologen in Oryza sativa.International journal of molecular sciences · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The HY1 gene of Arabidopsis encodes a plastid heme oxygenase (AtHO1) required for the synthesis of the chromophore of the phytochrome family of plant photoreceptors. To determine the enzymatic properties of plant heme oxygenases, we have expressed the HY1 gene (without the plastid transit peptide) in Escherichia coli to produce an amino terminal fusion protein between AtHO1 and glutathione S-transferase. The fusion protein was soluble and expressed at high levels. Purified recombinant AtHO1, after glutathione S-transferase cleavage, is a hemoprotein that forms a 1:1 complex with heme. In the presence of reduced ferredoxin, AtHO1 catalyzed the formation of biliverdin IXalpha from heme with the concomitant production of carbon monoxide. Heme oxygenase activity could also be reconstituted using photoreduced ferredoxin generated through light irradiation of isolated thylakoid membranes, suggesting that ferredoxin may be the electron donor in vivo. In addition, AtHO1 required an iron chelator and second reductant, such as ascorbate, for full activity. These results show that the basic mechanism of heme cleavage has been conserved between plants and other organisms even though the function, subcellular localization, and cofactor requirements of heme oxygenases differ substantially.
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