ArticlePlant physiology2002
Characterization of Arabidopsis AtAMT2, a high-affinity ammonium transporter of the plasma membrane.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 170 citations in OpenAlex.
- Article
- An Ammonium Transporter Gene Contributes to the Aggressiveness of the Dutch Elm Disease PathogenJournal of fungi (Basel, Switzerland) · 2026Article
- Genome-Wide Analysis of Ammonium Transporter Genes in Flowering Chinese Cabbage and Functional Insights into BcAMT1.1 Under Low-Nitrogen Conditions.Plants (Basel, Switzerland) · 2025Article
- Transcriptome Analysis of Macrophytes'Plants (Basel, Switzerland) · 2023Article
- Research Progress in High-Efficiency Utilization of Nitrogen in Rapeseed.International journal of molecular sciences · 2023Review
- Genome-Wide Identification and Expression Analysis of the Ammonium Transporter Family Genes in Soybean.International journal of molecular sciences · 2023Article
- Genome-wide identification, expression profiling, and functional analysis of ammonium transporter 2 (AMT2) gene family in cassava (Frontiers in genetics · 2023Article
- An integrated nitrogen utilization gene network and transcriptome analysis reveal candidate genes in response to nitrogen deficiency inFrontiers in plant science · 2023Article
- The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics.Journal of fungi (Basel, Switzerland) · 2022Article
- Genome-Wide Identification of AMT2-Type Ammonium Transporters Reveal That CsAMT2.2 and CsAMT2.3 Potentially Regulate NHInternational journal of molecular sciences · 2022Article
- The mycorrhiza-specific ammonium transporter ZmAMT3;1 mediates mycorrhiza-dependent nitrogen uptake in maize roots.The Plant cell · 2022Article
- The Roles of Phosphorus and Nitrogen Nutrient Transporters in the Arbuscular Mycorrhizal Symbiosis.International journal of molecular sciences · 2022Review
- Nitrogen in plants: from nutrition to the modulation of abiotic stress adaptation.Stress biology · 2022Review
- Differences and related physiological mechanisms in effects of ammonium on the invasive plantFrontiers in plant science · 2022Article
- Understanding plant-microbe interaction of rice and soybean with two contrasting diazotrophic bacteria through comparative transcriptome analysis.Frontiers in plant science · 2022Article
- Functional characterization of the sugarcane (Frontiers in plant science · 2022Article
- Adaptation of cucumber seedlings to low temperature stress by reducing nitrate to ammonium during it's transportation.BMC plant biology · 2021Article
- Article
- Ammonium Accumulation Caused by Reduced Tonoplast V-ATPase Activity inInternational journal of molecular sciences · 2020Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
8 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
AtAMT2 is an ammonium transporter that is only distantly related to the five members of the AtAMT1 family of high-affinity ammonium transporters in Arabidopsis. The short-lived radioactive ion (13)NH(4)(+) was used to show that AtAMT2, expressed in yeast (Saccharomyces cerevisiae), is a high-affinity transporter with a K(m) for ammonium of about 20 microM. Changes in external pH between 5.0 and 7.5 had little effect on the K(m) for ammonium, indicating that NH(4)(+), not NH(3), is the substrate for AtAMT2. The AtAMT2 gene was expressed in all organs of Arabidopsis and was subject to nitrogen (N) regulation, at least in roots where expression was partially repressed by high concentrations of ammonium nitrate and derepressed in the absence of external N. Although expression of AtAMT2 in shoots responded little to changes in root N status, transcript levels in leaves declined under high CO(2) conditions. Transient expression of an AtAMT2-green fluorescent protein fusion protein in Arabidopsis leaf epidermal cells indicated a plasma membrane location for the AtAMT2 protein. Thus, AtAMT2 is likely to play a significant role in moving ammonium between the apoplast and symplast of cells throughout the plant. However, a dramatic reduction in the level of AtAMT2 transcript brought about by dsRNA interference with gene expression had no obvious effect on plant growth or development, under the conditions tested.
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