ArticlePlant physiology2002
Down-regulation of caffeic acid o-methyltransferase in maize revisited using a transgenic approach.
Article in Plant physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed, 178 citations in OpenAlex.
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- Breeding Targets to Improve Biomass Quality in Miscanthus.Molecules (Basel, Switzerland) · 2021Review
- Genome-wide analysis of general phenylpropanoid and monolignol-specific metabolism genes in sugarcane.Functional & integrative genomics · 2021Article
- Tricin Biosynthesis and Bioengineering.Frontiers in plant science · 2021Review
- Overexpression of ferulate 5-hydroxylase increases syringyl units in Sorghum bicolor.Plant molecular biology · 2020Article
- Identification of genes from the general phenylpropanoid and monolignol-specific metabolism in two sugarcane lignin-contrasting genotypes.Molecular genetics and genomics : MGG · 2020Article
- The lignin toolbox of the model grass Setaria viridis.Plant molecular biology · 2019Article
- CtACO1 Overexpression Resulted in the Alteration of the Flavonoids Profile of Safflower.Molecules (Basel, Switzerland) · 2019Article
- RNAi-suppression of barley caffeic acid O-methyltransferase modifies lignin despite redundancy in the gene family.Plant biotechnology journal · 2019Article
- TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield.Plant biotechnology journal · 2018Article
- Stacking of a low-lignin trait with an increased guaiacyl and 5-hydroxyguaiacyl unit trait leads to additive and synergistic effects on saccharification efficiency inBiotechnology for biofuels · 2018Article
- Profiling of the Major Phenolic Compounds and Their Biosynthesis Genes inTheScientificWorldJournal · 2018Article
- Comparative transcriptome analysis of oil palm flowers reveals an EAR-motif-containing R2R3-MYB that modulates phenylpropene biosynthesis.BMC plant biology · 2017Article
- Silencing CHALCONE SYNTHASE in Maize Impedes the Incorporation of Tricin into Lignin and Increases Lignin Content.Plant physiology · 2017Article
- Modification of Monolignol Biosynthetic Pathway in Jute: Different Gene, Different Consequence.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic maize (Zea mays) plants were generated with a construct harboring a maize caffeic acid O-methyltransferase (COMT) cDNA in the antisense (AS) orientation under the control of the maize Adh1 (alcohol dehydrogenase) promoter. Adh1-driven beta-glucuronidase expression was localized in vascular tissues and lignifying sclerenchyma, indicating its suitability in transgenic experiments aimed at modifying lignin content and composition. One line of AS plants, COMT-AS, displayed a significant reduction in COMT activity (15%-30% residual activity) and barely detectable amounts of COMT protein as determined by western-blot analysis. In this line, transgenes were shown to be stably integrated in the genome and transmitted to the progeny. Biochemical analysis of COMT-AS showed: (a) a strong decrease in Klason lignin content at the flowering stage, (b) a decrease in syringyl units, (c) a lower p-coumaric acid content, and (d) the occurrence of unusual 5-OH guaiacyl units. These results are reminiscent of some characteristics already observed for the maize bm3 (brown-midrib3) mutant, as well as for COMT down-regulated dicots. However, as compared with bm3, COMT down-regulation in the COMT-AS line is less severe in that it is restricted to sclerenchyma cells. To our knowledge, this is the first time that an AS strategy has been applied to modify lignin biosynthesis in a grass species.
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