ArticleAnnals of the rheumatic diseases2003
Time between onset of apoptosis and release of nucleosomes from apoptotic cells: putative implications for systemic lupus erythematosus.
Article in Annals of the rheumatic diseases, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 98 citations in OpenAlex.
- Early prediction of therapy response in patients with acute myeloid leukemia by nucleosomal DNA fragments.BMC cancer · 2006Trial
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- Plasma Exchange Therapy Using Solvent Detergent-Treated Plasma: An Observational Pilot Study on Complement, Neutrophil and Endothelial Cell Activation in a Case Series of Patients Suffering from Atypical Hemolytic Uremic Syndrome.Transfusion medicine and hemotherapy : offizielles Organ der Deutschen Gesellschaft fur Transfusionsmedizin und Immunhamatologie · 2022Article
- Extra-nuclear histones: origin, significance and perspectives.Molecular and cellular biochemistry · 2022Review
- A New Perspective on the Origin of DNA Double-Strand Breaks and Its Implications for Ageing.Genes · 2021Review
- Cytokine Storm as a Cellular Response to dsDNA Breaks: A New Proposal.Frontiers in immunology · 2021Review
- Elevated Plasma Histone H4 Levels Are an Important Risk Factor in the Development of Septic CardiomyopathyBalkan medical journal · 2020Article
- Interactions of high mobility group box protein 1 (HMGB1) with nucleic acids: Implications in DNA repair and immune responses.DNA repair · 2019Review
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- The impact of proliferation-migration tradeoffs on phenotypic evolution in cancer.Scientific reports · 2019Article
- Prevention of radiation-induced bystander effects by agents that inactivate cell-free chromatin released from irradiated dying cells.Cell death & disease · 2018Article
- Spatial Heterogeneity and Evolutionary Dynamics Modulate Time to Recurrence in Continuous and Adaptive Cancer Therapies.Cancer research · 2018Article
- DNA and factor VII-activating protease protect against the cytotoxicity of histones.Blood advances · 2017Article
- Toll-like receptor 9 enhances bacterial clearance and limits lung consolidation in murine pneumonia caused by methicillin resistantMolecular medicine (Cambridge, Mass.) · 2016Article
- Circulating nucleic acids damage DNA of healthy cells by integrating into their genomes.Journal of biosciences · 2015Article
- Circulating nucleic acids: a new class of physiological mobile genetic elements.F1000Research · 2015Article
- Diabetes-independent increase of factor VII-activating protease activation in patients with Gram-negative sepsis (melioidosis).Journal of thrombosis and haemostasis : JTH · 2015Observational
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the kinetics of nucleosome leakage from apoptotic cells in an in vitro system and extrapolate the results to autoimmune disease, in particular systemic lupus erythematosus.
methodsA sensitive nucleosome enzyme linked immunosorbent assay (ELISA) was developed, using a monoclonal antibody (mAb) against histone 3 and an mAb against nucleosomes. Nucleosome release during apoptotic cell death was studied in Jurkat cells. AnnexinV binding (early apoptosis) and propidium iodide positivity (late apoptosis) of the cells were compared with nucleosome release at different times after apoptosis induction.
resultsNucleosomes appeared in culture supernatant of Jurkat cells 24 to 48 hours after apoptosis induction, when the cells had been late apoptotic for more than 12 hours.
conclusionNucleosomes are released from late apoptotic Jurkat cells, with a 12 hour delay from the appearance of AnnexinV binding cells. This result suggests that in vivo scavenger mechanisms have 12 hours to remove apoptotic material from the circulation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.