ArticleJournal of virology2003
Regulation of minute virus of mice NS1 replicative functions by atypical PKClambda in vivo.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Design and Characterization of Mutated Variants of the Oncotoxic Parvoviral Protein NS1.Viruses · 2023Article
- Identification and genomic characterization of bovine parvovirus 1 in yaks.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2021Article
- Cancer Treatment Goes Viral: Using Viral Proteins to Induce Tumour-Specific Cell Death.Cancers · 2019Review
- Molecular Properties and Evolutionary Origins of a Parvovirus-Derived Myosin Fusion Gene in Guinea Pigs.Journal of virology · 2019Article
- DNA Binding and Cleavage by the Human Parvovirus B19 NS1 Nuclease Domain.Biochemistry · 2016Article
- Viral genes as oncolytic agents for cancer therapy.Cellular and molecular life sciences : CMLS · 2015Review
- PKCη/Rdx-driven phosphorylation of PDK1: a novel mechanism promoting cancer cell survival and permissiveness for parvovirus-induced lysis.PLoS pathogens · 2015Article
- An in-frame deletion in the NS protein-coding sequence of parvovirus H-1PV efficiently stimulates export and infectivity of progeny virions.Journal of virology · 2012Article
- Activation of a helper and not regulatory human CD4+ T cell response by oncolytic H-1 parvovirus.PloS one · 2012Article
- Article
- Ezrin-radixin-moesin family proteins are involved in parvovirus replication and spreading.Journal of virology · 2009Article
- Vesicular egress of non-enveloped lytic parvoviruses depends on gelsolin functioning.PLoS pathogens · 2008Article
- Homology modeling and examination of the effect of the D92E mutation on the H5N1 nonstructural protein NS1 effector domain.Journal of molecular modeling · 2007Article
- A viral adaptor protein modulating casein kinase II activity induces cytopathic effects in permissive cells.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- NS1 interaction with CKII alpha: novel protein complex mediating parvovirus-induced cytotoxicity.Journal of virology · 2006Article
- Expression profiling of human hepatoma cells reveals global repression of genes involved in cell proliferation, growth, and apoptosis upon infection with parvovirus H-1.Journal of virology · 2005Article
- NS-3 protein of the Junonia coenia densovirus is essential for viral DNA replication in an Ld 652 cell line and Spodoptera littoralis larvae.Journal of virology · 2004Article
- Modulation of minute virus of mice cytotoxic activities through site-directed mutagenesis within the NS coding region.Journal of virology · 2003Article
- Novel PKCeta is required to activate replicative functions of the major nonstructural protein NS1 of minute virus of mice.Journal of virology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Minute virus of mice NS1 protein is a multifunctional phosphoprotein endowed with a variety of enzymatic and regulatory activities necessary for progeny virus particle production. To regulate all of its different functions in the course of a viral infection, NS1 has been proposed to be modulated by posttranslational modifications, in particular, phosphorylation. Indeed, it was shown that the NS1 phosphorylation pattern is altered during the infectious cycle and that the biochemical profile of the protein is dependent on the phosphorylation state of the polypeptide. Moreover, in vitro approaches have identified members of the protein kinase C (PKC) family, in particular, atypical PKC, as regulators of viral DNA replication through the phosphorylation of NS1 residues T435 and S473, thereby activating the protein for DNA unwinding activities. In order to substantiate these findings in vivo, we produced NS1 in the presence of a dominant-negative PKClambda mutant and characterized the purified protein in vitro. The NS1 protein produced under these conditions was found to be only partially phosphorylated and as a consequence to be deficient for viral DNA replication. However, it could be rescued for this viral function by treatment with recombinant activated PKClambda. Our data clearly demonstrate that NS1 is a target for PKClambda phosphorylation in vivo and that this modification is essential for the helicase activity of the viral polypeptide. In addition, the phosphorylation of NS1 at residues T435 and S473 appeared to occur mainly in the nucleus, providing further evidence for the involvement of PKClambda which, unlike PKCzeta, accumulates in the nuclear compartment of infected cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.