ArticleJournal of virology2003
A direct transposon insertion tool for modification and functional analysis of viral genomes.
Article in Journal of virology, 2003. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Article
- TransposonUltimate: software for transposon classification, annotation and detection.Nucleic acids research · 2022Article
- Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering.Nucleic acids research · 2018Article
- MuA-mediated in vitro cloning of circular DNA: transpositional autointegration and the effect of MuB.Molecular genetics and genomics : MGG · 2016Article
- An assay to monitor the activity of DNA transposition complexes yields a general quality control measure for transpositional recombination reactions.Mobile genetic elements · 2014Article
- Article
- Universal platform for quantitative analysis of DNA transposition.Mobile DNA · 2010Article
- Transposition-based method for the rapid generation of gene-targeting vectors to produce Cre/Flp-modifiable conditional knock-out mice.PloS one · 2009Article
- Bacteriophage Mu integration in yeast and mammalian genomes.Nucleic acids research · 2008Article
- High-precision mapping of protein protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform.Nucleic acids research · 2007Article
- SNP discovery by mismatch-targeting of Mu transposition.Nucleic acids research · 2007Article
- Genome characterization of lipid-containing marine bacteriophage PM2 by transposon insertion mutagenesis.Journal of virology · 2006Article
- Selection of novel vesicular stomatitis virus glycoprotein variants from a peptide insertion library for enhanced purification of retroviral and lentiviral vectors.Journal of virology · 2006Article
- A gene truncation strategy generating N- and C-terminal deletion variants of proteins for functional studies: mapping of the Sec1p binding domain in yeast Mso1p by a Mu in vitro transposition-based approach.Nucleic acids research · 2005Article
- Nonessential genes of phage phiYeO3-12 include genes involved in adaptation to growth on Yersinia enterocolitica serotype O:3.Journal of bacteriology · 2005Article
- Identification and mutational analysis of bacteriophage PRD1 holin protein P35.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advances in DNA transposition technology have recently generated efficient tools for various types of functional genetic analyses. We demonstrate here the power of the bacteriophage Mu-derived in vitro DNA transposition system for modification and functional characterization of a complete bacterial virus genome. The linear double-stranded DNA genome of Escherichia coli bacteriophage PRD1 was studied by insertion mutagenesis with reporter mini-Mu transposons that were integrated in vitro into isolated genomic DNA. After introduction into bacterial cells by electroporation, recombinant transposon-containing virus clones were identified by autoradiography or visual blue-white screening employing alpha-complementation of E. coli beta-galactosidase. Additionally, a modified transposon with engineered NotI sites at both ends was used to introduce novel restriction sites into the phage genome. Analysis of the transposon integration sites in the genomes of viable recombinant phage generated a functional map, collectively indicating genes and genomic regions essential and nonessential for virus propagation. Moreover, promoterless transposons defined the direction of transcription within several insert-tolerant genomic regions. These strategies for the analysis of viral genomes are of a general nature and therefore may be applied to functional genomics studies in all prokaryotic and eukaryotic cell viruses.
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Registered trials
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