ArticleEukaryotic cell2002
Hyphal tip-associated localization of Cdc42 is F-actin dependent in Candida albicans.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- The development of single-domain VHH nanobodies that target theMicrobiology spectrum · 2024Article
- Hyphal swelling induced in the phagosome of macrophages.Fungal biology · 2024Article
- Hyphal Growth inMicrobiology spectrum · 2023Article
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- Fun30 nucleosome remodeller regulates white-to-opaque switching inActa biochimica et biophysica Sinica · 2023Article
- Sterols and Sphingolipids as New Players in Cell Wall Building and Apical Growth ofPlants (Basel, Switzerland) · 2022Article
- From Jekyll to Hyde: The Yeast-Hyphal Transition ofPathogens (Basel, Switzerland) · 2021Review
- Article
- The regulation of hyphae growth inVirulence · 2020Review
- Network analysis of hyphae forming proteins inHeliyon · 2019Article
- Regulation ofJournal of fungi (Basel, Switzerland) · 2019Review
- Candida albicans Hyphae: From Growth Initiation to Invasion.Journal of fungi (Basel, Switzerland) · 2018Review
- Spontaneous Cdc42 polarization independent of GDI-mediated extraction and actin-based trafficking.PLoS biology · 2015Article
- Cdc42 GTPase dynamics control directional growth responses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Rsr1 focuses Cdc42 activity at hyphal tips and promotes maintenance of hyphal development in Candida albicans.Eukaryotic cell · 2013Article
- A steep phosphoinositide bis-phosphate gradient forms during fungal filamentous growth.The Journal of cell biology · 2012Article
- Review
- Roles of Ras1 membrane localization during Candida albicans hyphal growth and farnesol response.Eukaryotic cell · 2011Article
- Growth of Candida albicans hyphae.Nature reviews. Microbiology · 2011Review
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2 authors.
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Abstract
The rho-type GTPase Cdc42 is important for the establishment and maintenance of eukaryotic cell polarity. To examine whether Cdc42 is regulated during the yeast-to-hypha transition in Candida albicans, we constructed a green fluorescence protein (GFP)-Cdc42 fusion under the ACT1 promoter and observed its localization in live C. albicans cells. As in Saccharomyces cerevisiae, GFP-Cdc42 was observed around the entire periphery of the cell. In yeast-form cells of C. albicans, it clustered to the tips and sides of small buds as well as to the mother-daughter neck region of large-budded cells. Upon hyphal induction, GFP-Cdc42 clustered to the site of hyphal evagination and remained at the tips of the hyphae. This temporal and spatial localization of Cdc42 suggests that its activity is regulated during the yeast-to-hypha transition. In addition to the accumulation at the hyphal tip, GFP-Cdc42 was also seen as a band within the hyphal tube in cells that had undergone nuclear separation. With the F-actin-assembly inhibitor latrunculin A, we found that GFP-Cdc42 accumulation at the bud site in yeast-form cells is F-actin independent, whereas GFP-Cdc42 accumulation at the hyphal tip requires F-actin. Furthermore, disruption of the F-actin cytoskeleton impaired the transcriptional induction of hypha-specific genes. Therefore, hypha formation resembles mating in Saccharomyces cerevisiae in that both require F-actin for GFP-Cdc42 localization and efficient signaling.
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