ArticleMolecular biology of the cell2002
Microtubule asymmetry during neutrophil polarization and migration.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Cytoskeleton-mediated autophagy regulation in neuroimmune contexts: molecular mechanisms and functional perspectives.Frontiers in immunology · 2026Review
- Multiple centrosomes enhance migration and immune cell effector functions of mature dendritic cells.The Journal of cell biology · 2022Article
- Centrosome Positioning in MigratingCells · 2022Article
- Article
- Microtubule destabilization is a critical checkpoint of chemotaxis and transendothelial migration in melanoma cells but not in T cells.Cell adhesion & migration · 2021Article
- Dynamic Microtubule Arrays in Leukocytes and Their Role in Cell Migration and Immune Synapse Formation.Frontiers in cell and developmental biology · 2021Review
- Neutrophils-From Bone Marrow to First-Line Defense of the Innate Immune System.Frontiers in immunology · 2021Review
- Microtubules in cell migration.Essays in biochemistry · 2019Review
- Article
- The Neutrophil Nucleus: An Important Influence on Neutrophil Migration and Function.Frontiers in immunology · 2018Review
- ROS and glutathionylation balance cytoskeletal dynamics in neutrophil extracellular trap formation.The Journal of cell biology · 2017Article
- Live imaging reveals distinct modes of neutrophil and macrophage migration within interstitial tissues.Journal of cell science · 2017Article
- Leading from the Back: The Role of the Uropod in Neutrophil Polarization and Migration.Developmental cell · 2016Review
- Critical behavior of subcellular density organization during neutrophil activation and migration.Cellular and molecular bioengineering · 2015Article
- Aquaporin-9-expressing neutrophils are required for the establishment of contact hypersensitivity.Scientific reports · 2015Article
- Midline 1 controls polarization and migration of murine cytotoxic T cells.Immunity, inflammation and disease · 2014Article
- Wnt5a directs polarized calcium gradients by recruiting cortical endoplasmic reticulum to the cell trailing edge.Developmental cell · 2013Article
- Identifying network motifs that buffer front-to-back signaling in polarized neutrophils.Cell reports · 2013Article
- The role of microtubules in neutrophil polarity and migration in live zebrafish.Journal of cell science · 2012Article
- Cdc42 regulates neutrophil migration via crosstalk between WASp, CD11b, and microtubules.Blood · 2012Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The development of cell polarity in response to chemoattractant stimulation in human polymorphonuclear neutrophils (PMNs) is characterized by the rapid conversion from round to polarized morphology with a leading lamellipod at the front and a uropod at the rear. During PMN polarization, the microtubule (MT) array undergoes a dramatic reorientation toward the uropod that is maintained during motility and does not require large-scale MT disassembly or cell adhesion to the substratum. MTs are excluded from the leading lamella during polarization and motility, but treatment with a myosin light chain kinase inhibitor (ML-7) or the actin-disrupting drug cytochalasin D causes an expansion of the MT array and penetration of MTs into the lamellipod. Depolymerization of the MT array before stimulation caused 10% of the cells to lose their polarity by extending two opposing lateral lamellipodia. These multipolar cells showed altered localization of a leading lamella-specific marker, talin, and a uropod-specific marker, CD44. In summary, these results indicate that F-actin- and myosin II-dependent forces lead to the development and maintenance of MT asymmetry that may act to reinforce cell polarity during PMN migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.