ArticleMolecular biology of the cell2002
Requirement of the N-terminal extension for vacuolar trafficking and transport activity of yeast Ycf1p, an ATP-binding cassette transporter.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 60 citations in OpenAlex.
- Structural insights into toxicant export mediated by ABCC2 in Arabidopsis thaliana.Nature communications · 2025Article
- Structure of a dimeric full-length ABC transporter.Nature communications · 2024Article
- Cadmium transport by mammalian ATP-binding cassette transporters.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2024Review
- Multilevel Regulation of Membrane Proteins in Response to Metal and Metalloid Stress: A Lesson from Yeast.International journal of molecular sciences · 2024Review
- Structure of Ycf1p reveals the transmembrane domain TMD0 and the regulatory region of ABCC transporters.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Pre-emptive Quality Control of a Misfolded Membrane Protein by Ribosome-Driven Effects.Current biology : CB · 2020Article
- ABC Transporter Genes Show Upregulated Expression in Drug-Resistant Clinical Isolates ofFrontiers in microbiology · 2019Article
- N-Terminal Extension and C-Terminal Domains Are Required for ABCB6/HMT-1 Protein Interactions, Function in Cadmium Detoxification, and Localization to the Endosomal-Recycling System inFrontiers in physiology · 2018Article
- New insights into the roles of the N-terminal region of the ABCC6 transporter.Journal of bioenergetics and biomembranes · 2016Article
- Role of the N-terminal transmembrane domain in the endo-lysosomal targeting and function of the human ABCB6 protein.The Biochemical journal · 2015Article
- YCF1-mediated cadmium resistance in yeast is dependent on copper metabolism and antioxidant enzymes.Antioxidants & redox signaling · 2014Article
- Identification of a human ABCC10 orthologue in Catharanthus roseus reveals a U12-type intron determinant for the N-terminal domain feature.Journal of genetics · 2014Article
- Casein kinase 2α regulates multidrug resistance-associated protein 1 function via phosphorylation of Thr249.Molecular pharmacology · 2012Article
- The Rho1 GTPase acts together with a vacuolar glutathione S-conjugate transporter to protect yeast cells from oxidative stress.Genetics · 2011Article
- Plant ABC Transporters.The arabidopsis book · 2011Article
- Suppression of Ycf1p function by Cka1p-dependent phosphorylation is attenuated in response to salt stress.FEMS yeast research · 2010Article
- Article
- Detoxification of multiple heavy metals by a half-molecule ABC transporter, HMT-1, and coelomocytes of Caenorhabditis elegans.PloS one · 2010Article
- GintABC1 encodes a putative ABC transporter of the MRP subfamily induced by Cu, Cd, and oxidative stress in Glomus intraradices.Mycorrhiza · 2010Article
- abc3+ encodes an iron-regulated vacuolar ABC-type transporter in Schizosaccharomyces pombe.Eukaryotic cell · 2010Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Ycf1p is the prototypical member of the yeast multidrug resistance-associated protein (MRP) subfamily of ATP-binding cassette (ABC) transporters. Ycf1p resides in the vacuolar membrane and mediates glutathione-dependent transport processes that result in resistance to cadmium and other xenobiotics. A feature common to many MRP proteins that distinguishes them from other ABC transporters is the presence of a hydrophobic N-terminal extension (NTE), whose function is not clearly established. The NTE contains a membrane spanning domain (MSD0) with five transmembrane spans and a cytosolic linker region (L0). The goal of this study was to determine the functional significance of the NTE of Ycf1p by examining the localization and functional properties of Ycf1p partial molecules, expressed either singly or together. We show that MSD0 plays a critical role in the vacuolar membrane trafficking of Ycf1p, whereas L0 is dispensable for localization. On the other hand, L0 is required for transport function, as determined by monitoring cadmium resistance. We also examine an unusual aspect of Ycf1p biology, namely, the posttranslational proteolytic processing that occurs within a lumenal loop of Ycf1p. Processing is shown to be Pep4p dependent and thus serves as a convenient marker for proper vacuolar localization. The processed fragments associate with each other, suggesting that these natural cleavage products contribute together to Ycf1p function.
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