ArticleMolecular biology of the cell2002
Identification of the nuclear localization signal in Xenopus cyclin E and analysis of its role in replication and mitosis.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Cyclin E/CDK2: DNA Replication, Replication Stress and Genomic Instability.Frontiers in cell and developmental biology · 2021Review
- Molecular crosstalk between cancer and neurodegenerative diseases.Cellular and molecular life sciences : CMLS · 2020Review
- Inhibition of Cyclin-dependent Kinase 2 Signaling Prevents Liver Ischemia and Reperfusion Injury.Transplantation · 2019Article
- Low-Molecular-Weight Cyclin E in Human Cancer: Cellular Consequences and Opportunities for Targeted Therapies.Cancer research · 2018Review
- Novel Molecular Markers for Breast Cancer.Biomarkers in cancer · 2016Review
- DEC1 regulates breast cancer cell proliferation by stabilizing cyclin E protein and delays the progression of cell cycle S phase.Cell death & disease · 2015Article
- Regulation of Greatwall kinase by protein stabilization and nuclear localization.Cell cycle (Georgetown, Tex.) · 2014Article
- Oncogenic Ras suppresses Cdk1 in a complex manner during the incubation of activated Xenopus egg extracts.Archives of biochemistry and biophysics · 2013Article
- In the wrong place at the wrong time: does cyclin mislocalization drive oncogenic transformation?Nature reviews. Cancer · 2013Review
- Breast cancer cell uptake of the inflammatory mediator neutrophil elastase triggers an anticancer adaptive immune response.Cancer research · 2012Article
- Developmental downregulation of Xenopus cyclin E is phosphorylation and nuclear import dependent and is mediated by ubiquitination.Developmental biology · 2011Article
- MCM-BP regulates unloading of the MCM2-7 helicase in late S phase.Genes & development · 2011Article
- Clusters, factories and domains: The complex structure of S-phase comes into focus.Cell cycle (Georgetown, Tex.) · 2010Article
- Nuclear targeting of cyclin-dependent kinase 2 reveals essential roles of cyclin-dependent kinase 2 localization and cyclin E in vitamin D-mediated growth inhibition.Endocrinology · 2010Article
- Replication factory activation can be decoupled from the replication timing program by modulating Cdk levels.The Journal of cell biology · 2010Article
- MAP kinase dependent cyclinE/cdk2 activity promotes DNA replication in early sea urchin embryos.Developmental biology · 2009Article
- Towards a systems biology approach to mammalian cell cycle: modeling the entrance into S phase of quiescent fibroblasts after serum stimulation.BMC bioinformatics · 2009Article
- Cyclin E deregulation is an early event in the development of breast cancer.Breast cancer research and treatment · 2009Article
- Altered subcellular localization of tumor-specific cyclin E isoforms affects cyclin-dependent kinase 2 complex formation and proteasomal regulation.Cancer research · 2009Article
- Replication initiation complex formation in the absence of nuclear function in Xenopus.Nucleic acids research · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Cyclin-dependent kinase (Cdk)2/cyclin E is imported into nuclei assembled in Xenopus egg extracts by a pathway that requires importin-alpha and -beta. Here, we identify a basic nuclear localization sequence (NLS) in the N-terminus of Xenopus cyclin E. Mutation of the NLS eliminated nuclear accumulation of both cyclin E and Cdk2, and such versions of cyclin E were unable to trigger DNA replication. Addition of a heterologous NLS from SV40 large T antigen restored both nuclear targeting of Cdk2/cyclin E and DNA replication. We present evidence indicating that Cdk2/cyclin E complexes must become highly concentrated within nuclei to support replication and find that cyclin A can trigger replication at much lower intranuclear concentrations. We confirmed that depletion of endogenous cyclin E increases the concentration of cyclin B necessary to promote entry into mitosis. In contrast to its inability to promote DNA replication, cyclin E lacking its NLS was able to cooperate with cyclin B in promoting mitotic entry.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.