ArticleMolecular biology of the cell2002
EB1 targets to kinetochores with attached, polymerizing microtubules.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 90 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
90 citing papers in PubMed, 176 citations in OpenAlex.
- The microtubule GTP-tubulin cap size is modulated during cell division.Molecular biology of the cell · 2026Article
- The microtubule GTP-tubulin cap size is modulated during cell division.bioRxiv : the preprint server for biology · 2026Article
- Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch.Science advances · 2025Article
- α-tubulin detyrosination fine-tunes kinetochore-microtubule attachments.Nature communications · 2024Article
- Direct targeting of host microtubule and actin cytoskeletons by a chlamydial pathogenic effector protein.Journal of cell science · 2024Article
- Article
- Mechanical coupling coordinates microtubule growth.bioRxiv : the preprint server for biology · 2023Article
- Systematic analysis of microtubule plus-end networks defines EB-cargo complexes critical for mitosis in budding yeast.Molecular biology of the cell · 2023Article
- Evidence for a HURP/EB free mixed-nucleotide zone in kinetochore-microtubules.Nature communications · 2022Article
- Control of division in Chlamydomonas by cyclin B/CDKB1 and the anaphase-promoting complex.PLoS genetics · 2022Article
- Optogenetic EB1 inactivation shortens metaphase spindles by disrupting cortical force-producing interactions with astral microtubules.Current biology : CB · 2022Article
- Gradual compaction of the central spindle decreases its dynamicity in PRC1 and EB1 gene-edited cells.Life science alliance · 2021Article
- Moonlighting of mitotic regulators in cilium disassembly.Cellular and molecular life sciences : CMLS · 2021Review
- Article
- Microtubules: Evolving roles and critical cellular interactions.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Interaction of microtubules and actin during the post-fusion phase of exocytosis.Scientific reports · 2019Article
- Augmin accumulation on long-lived microtubules drives amplification and kinetochore-directed growth.The Journal of cell biology · 2019Article
- EB1 contributes to microtubule bundling and organization, along with root growth, inBiology open · 2018Article
- Article
- Microtubule Dynamics Scale with Cell Size to Set Spindle Length and Assembly Timing.Developmental cell · 2018Article
30 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Microtubule polymerization dynamics at kinetochores is coupled to chromosome movements, but its regulation there is poorly understood. The plus end tracking protein EB1 is required both for regulating microtubule dynamics and for maintaining a euploid genome. To address the role of EB1 in aneuploidy, we visualized its targeting in mitotic PtK1 cells. Fluorescent EB1, which localized to polymerizing ends of astral and spindle microtubules, was used to track their polymerization. EB1 also associated with a subset of attached kinetochores in late prometaphase and metaphase, and rarely in anaphase. Localization occurred in a narrow crescent, concave toward the centromere, consistent with targeting to the microtubule plus end-kinetochore interface. EB1 did not localize to kinetochores lacking attached kinetochore microtubules in prophase or early prometaphase, or upon nocodazole treatment. By time lapse, EB1 specifically targeted to kinetochores moving antipoleward, coupled to microtubule plus end polymerization, and not during plus end depolymerization. It localized independently of spindle bipolarity, the spindle checkpoint, and dynein/dynactin function. EB1 is the first protein whose targeting reflects kinetochore directionality, unlike other plus end tracking proteins that show enhanced kinetochore binding in the absence of microtubules. Our results suggest EB1 may modulate kinetochore microtubule polymerization and/or attachment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.