ArticleMolecular biology of the cell2002
Entamoeba histolytica expressing a dominant negative N-truncated light subunit of its gal-lectin are less virulent.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 45 citations in OpenAlex.
- Evaluation of the PEJournal of immunology research · 2021Article
- Interaction between human mucins and parasite glycoproteins: the role of lectins and glycosidases in colonization by intestinal protozoa.Revista do Instituto de Medicina Tropical de Sao Paulo · 2020Article
- Entamoeba histolytica and pathogenesis: A calcium connection.PLoS pathogens · 2020Review
- Functional Characterization of an Interferon Gamma Receptor-Like Protein on Entamoeba histolytica.Infection and immunity · 2019Article
- Review
- Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins.PloS one · 2017Article
- Entamoeba histolytica: adhesins and lectins in the trophozoite surface.Molecules (Basel, Switzerland) · 2015Review
- Destabilization domain approach adapted for regulated protein expression in the protozoan parasite Entamoeba histolytica.International journal for parasitology · 2014Article
- The genome and transcriptome of the enteric parasite Entamoeba invadens, a model for encystation.Genome biology · 2013Article
- Cysteine protease-binding protein family 6 mediates the trafficking of amylases to phagosomes in the enteric protozoan Entamoeba histolytica.Infection and immunity · 2013Article
- Common pathways for receptor-mediated ingestion of Escherichia coli and LDL cholesterol by Entamoeba histolytica regulated in part by transmembrane kinase 39.International journal for parasitology · 2012Article
- Novel transmembrane receptor involved in phagosome transport of lysozymes and β-hexosaminidase in the enteric protozoan Entamoeba histolytica.PLoS pathogens · 2012Article
- New insights into host-pathogen interactions during Entamoeba histolytica liver infection.European journal of microbiology & immunology · 2011Review
- Serum-dependent selective expression of EhTMKB1-9, a member of Entamoeba histolytica B1 family of transmembrane kinases.PLoS pathogens · 2010Article
- Host-microbe interactions and defense mechanisms in the development of amoebic liver abscesses.Clinical microbiology reviews · 2009Review
- Entamoeba histolytica phagocytosis of human erythrocytes involves PATMK, a member of the transmembrane kinase family.PLoS pathogens · 2008Article
- Identification of an Entamoeba histolytica serine-, threonine-, and isoleucine-rich protein with roles in adhesion and cytotoxicity.Eukaryotic cell · 2007Article
- Transcriptional gene silencing reveals two distinct groups of Entamoeba histolytica Gal/GalNAc-lectin light subunits.Eukaryotic cell · 2007Article
- EhLimA, a novel LIM protein, localizes to the plasma membrane in Entamoeba histolytica.Eukaryotic cell · 2007Article
- Transcriptional silencing of multiple genes in trophozoites of Entamoeba histolytica.PLoS pathogens · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 260-kDa heterodimeric Gal/GalNAc-specific Lectin (Gal-lectin) of Entamoeba histolytica dissociates under reducing conditions into a heavy (hgl, 170 kDa) and a light subunit (lgl, 35 kDa). We have previously shown that inhibition of expression of the 35-kDa subunit by antisense RNA causes a decrease in virulence. To further understand the role of the light subunit of the Gal-lectin in pathogenesis, amoebae were transfected with plasmids encoding intact, mutated, and truncated forms of the light subunit lgl1 gene. A transfectant in which the 55 N-terminal amino acids of the lgl were removed, overproduced an N-truncated lgl protein (32 kDa), which replaced most of the native 35-kDa lgl in the formation of the Gal-lectin heterodimeric complex and exerted a dominant negative effect. Amoebae transfected with this construct showed a significant decrease in their ability to adhere to and kill mammalian cells as well as in their capacity to form rosettes with and to phagocytose erythrocytes. In addition, immunofluorescence confocal microscopy of this transfectant with anti-Gal-lectin antibodies showed an impaired ability to cap. These results indicate that the light subunit has a role in enabling the clustering of Gal-lectin complexes and that its N-truncation affects this function, which is required for virulence.
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