ArticleMolecular biology of the cell2002
Specific heterodimer formation is a prerequisite for uroplakins to exit from the endoplasmic reticulum.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Who cites it
56 citing papers in PubMed, 115 citations in OpenAlex.
- Preclinical Models of Bladder Cancer: Barrier, Metabolic, and Translational Susceptibility.Pharmaceuticals (Basel, Switzerland) · 2026Review
- Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Cryo-EM elucidates the uroplakin complex structure within liquid-crystalline lipids in the porcine urothelial membrane.Communications biology · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.bioRxiv : the preprint server for biology · 2023Article
- Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM.Research square · 2023Article
- Uroplakin 1a Knockout Mice Display Marginal Reduction in Fecundity, Decreased Bacterial Clearance Capacity, and Drastic Changes in the Testicular Transcriptome.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- The Golgi complex: An organelle that determines urothelial cell biology in health and disease.Histochemistry and cell biology · 2022Review
- The urothelium: a multi-faceted barrier against a harsh environment.Mucosal immunology · 2022Review
- Development, regeneration and tumorigenesis of the urothelium.Development (Cambridge, England) · 2022Review
- Immunohistochemistry as a paramount tool in research of normal urothelium, bladder cancer and bladder pain syndrome.European journal of histochemistry : EJH · 2021Review
- The Urothelium: Life in a Liquid Environment.Physiological reviews · 2020Review
- Changes in uroplakin expression in the urothelium of patients with ulcerative interstitial cystitis/bladder pain syndrome.Investigative and clinical urology · 2020Article
- Toward the understanding of biology of oocyte life cycle inReproductive medicine and biology · 2020Review
- Mitochondrial lipid droplet formation as a detoxification mechanism to sequester and degrade excessive urothelial membranes.Molecular biology of the cell · 2019Article
- Uroplakins play conserved roles in egg fertilization and acquired additional urothelial functions during mammalian divergence.Molecular biology of the cell · 2018Article
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- Uroplakin traffic through the Golgi apparatus induces its fragmentation: new insights from novel in vitro models.Scientific reports · 2017Article
- New insights into the tetraspanin Tspan5 using novel monoclonal antibodies.The Journal of biological chemistry · 2017Article
- The Tetraspanin-Associated Uroplakins Family (UPK2/3) Is Evolutionarily Related to PTPRQ, a Phosphotyrosine Phosphatase Receptor.PloS one · 2017Article
- Sequential and compartmentalized action of Rabs, SNAREs, and MAL in the apical delivery of fusiform vesicles in urothelial umbrella cells.Molecular biology of the cell · 2016Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
Much of the lower urinary tract, including the bladder, is lined by a stratified urothelium forming a highly differentiated, superficial umbrella cell layer. The apical plasma membrane as well as abundant cytoplasmic fusiform vesicles of the umbrella cells is covered by two-dimensional crystals that are formed by four membrane proteins named uroplakins (UPs) Ia, Ib, II, and III. UPs are synthesized on membrane-bound polysomes, and after several co- and posttranslational modifications they assemble into planar crystals in a post-Golgi vesicular compartment. Distension of the bladder may cause fusiform vesicles to fuse with the apical plasma membrane. We have investigated the early stages of uroplakin assembly by expressing the four uroplakins in 293T cells. Transfection experiments showed that, when expressed individually, only UPIb can exit from the endoplasmic reticulum (ER) and move to the plasma membrane, whereas UPII and UPIII reach the plasma membrane only when they form heterodimeric complexes with UPIa and UPIb, respectively. Heterodimer formation in the ER was confirmed by pulse-chase experiment followed by coimmunoprecipitation. Our results indicate that the initial building blocks for the assembly of crystalline uroplakin plaques are heterodimeric uroplakin complexes that form in the ER.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.