ArticleMolecular biology of the cell2002
Kar9p-independent microtubule capture at Bud6p cortical sites primes spindle polarity before bud emergence in Saccharomyces cerevisiae.
Article in Molecular biology of the cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- APC/CCdc20-mediated degradation of Clb4 prompts astral microtubule stabilization at anaphase onset.The Journal of cell biology · 2023Article
- Bil2 Is a Novel Inhibitor of the Yeast Formin Bnr1 Required for Proper Actin Cable Organization and Polarized Secretion.Frontiers in cell and developmental biology · 2021Article
- Integrated control of formin-mediated actin assembly by a stationary inhibitor and a mobile activator.The Journal of cell biology · 2018Article
- Nuclear movement in fungi.Seminars in cell & developmental biology · 2018Review
- Article
- Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.Molecular biology of the cell · 2011Article
- Actin-mediated delivery of astral microtubules instructs Kar9p asymmetric loading to the bud-ward spindle pole.Molecular biology of the cell · 2010Article
- Stable preanaphase spindle positioning requires Bud6p and an apparent interaction between the spindle pole bodies and the neck.Eukaryotic cell · 2007Article
- The yeast actin cytoskeleton: from cellular function to biochemical mechanism.Microbiology and molecular biology reviews : MMBR · 2006Review
- Differential contribution of Bud6p and Kar9p to microtubule capture and spindle orientation in S. cerevisiae.The Journal of cell biology · 2004Article
- Cdk1-Clb4 controls the interaction of astral microtubule plus ends with subdomains of the daughter cell cortex.Genes & development · 2004Article
- Eukaryotic cells and their cell bodies: Cell Theory revised.Annals of botany · 2004Review
- The differential roles of budding yeast Tem1p, Cdc15p, and Bub2p protein dynamics in mitotic exit.Molecular biology of the cell · 2004Article
- A conserved mechanism for Bni1- and mDia1-induced actin assembly and dual regulation of Bni1 by Bud6 and profilin.Molecular biology of the cell · 2004Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Spindle orientation is critical for accurate chromosomal segregation in eukaryotic cells. In the yeast Saccharomyces cerevisiae, orientation of the mitotic spindle is achieved by a program of microtubule-cortex interactions coupled to spindle morphogenesis. We previously implicated Bud6p in directing microtubule capture throughout this program. Herein, we have analyzed cells coexpressing GFP:Bud6 and GFP:Tub1 fusions, providing a kinetic view of Bud6p-microtubule interactions in live cells. Surprisingly, even during the G1 phase, microtubule capture at the recent division site and the incipient bud is dictated by Bud6p. These contacts are eliminated in bud6 delta cells but are proficient in kar9 delta cells. Thus, Bud6p cues microtubule capture, as soon as a new cell polarity axis is established independent of Kar9p. Bud6p increases the duration of interactions and promotes distinct modes of cortical association within the bud and neck regions. In particular, microtubule shrinkage and growth at the cortex rarely occur away from Bud6p sites. These are the interactions selectively impaired at the bud cortex in bud6 delta cells. Finally, interactions away from Bud6p sites within the bud differ from those occurring at the mother cell cortex, pointing to the existence of an independent factor controlling cortical contacts in mother cells after bud emergence.
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