ArticleProceedings of the National Academy of Sciences of the United States of America2002
Noninvasive real-time imaging of apoptosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
67 citing papers in PubMed, 248 citations in OpenAlex.
- A highly selective iron oxide-based imaging nanoparticle for long-term monitoring of drug-induced tumor cell apoptosis.Biomaterials science · 2021Article
- Protective effects of vitamin E on cadmium-induced apoptosis in rat testes.Naunyn-Schmiedeberg's archives of pharmacology · 2020Article
- Optical imaging with a novel cathepsin-activatable probe for enhanced detection of colorectal cancer.American journal of nuclear medicine and molecular imaging · 2019Article
- Advances in nanomaterial based optical biosensing and bioimaging of apoptosis via caspase-3 activity: a review.Mikrochimica acta · 2018Review
- Nanostructured Ti surfaces and retinoic acid/dexamethasone present a spatial framework for the maturation and amelogenesis of LS-8 cells.International journal of nanomedicine · 2018Article
- Caspase-3/-7-Specific Metabolic Precursor for Bioorthogonal Tracking of Tumor Apoptosis.Scientific reports · 2017Article
- Effects of Combined Simultaneous and Sequential Endostar and Cisplatin Treatment in a Mice Model of Gastric Cancer Peritoneal Metastases.Gastroenterology research and practice · 2017Article
- Molecular imaging with engineered physiology.Nature communications · 2016Article
- Dual-reporter Imaging and its Potential Application in Tracking Studies.Journal of fluorescence · 2016Article
- Article
- Small Molecule Active Site Directed Tools for Studying Human Caspases.Chemical reviews · 2015Review
- Design of a functional cyclic HSV1-TK reporter and its application to PET imaging of apoptosis.Nature protocols · 2015Article
- A cyclic HSV1-TK reporter for real-time PET imaging of apoptosis.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- A novel reporter system for molecular imaging and high-throughput screening of anticancer drugs.Chembiochem : a European journal of chemical biology · 2013Article
- Non-invasive molecular imaging for preclinical cancer therapeutic development.British journal of pharmacology · 2013Review
- Continuous, real-time bioimaging of chemical bioavailability and toxicology using autonomously bioluminescent human cell lines.Proceedings of SPIE--the International Society for Optical Engineering · 2013Article
- A biocompatible in vivo ligation reaction and its application for noninvasive bioluminescent imaging of protease activity in living mice.ACS chemical biology · 2013Article
- Longitudinal bioluminescence imaging of the dynamics of Doxorubicin induced apoptosis.Theranostics · 2013Article
- Biomarkers and molecular probes for cell death imaging and targeted therapeutics.Bioconjugate chemistry · 2012Review
- Imaging molecular pathways: reporter genes.Radiation research · 2012Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Strict coordination of proliferation and programmed cell death (apoptosis) is essential for normal physiology. An imbalance in these two opposing processes results in various diseases including AIDS, neurodegenerative disorders, myelodysplastic syndromes, ischemiareperfusion injury, cancer, autoimmune disease, among others. Objective and quantitative noninvasive imaging of apoptosis would be a significant advance for rapid and dynamic screening as well as validation of experimental therapeutic agents. Here, we report the development of a recombinant luciferase reporter molecule that when expressed in mammalian cells has attenuated levels of reporter activity. In cells undergoing apoptosis, a caspase-3-specific cleavage of the recombinant product occurs, resulting in the restoration of luciferase activity that can be detected in living animals with bioluminescence imaging. The ability to image apoptosis noninvasively and dynamically over time provides an opportunity for high-throughput screening of proapoptotic and antiapoptotic compounds and for target validation in vivo in both cell lines and transgenic animals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.