ArticleThe Plant cell2002
Host DNA replication is induced by geminivirus infection of differentiated plant cells.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 67 citations in OpenAlex.
- DNA Satellites Impact Begomovirus Diseases in a Virus-Specific Manner.International journal of molecular sciences · 2025Review
- SEGS-1 episomes generated during cassava mosaic disease enhance disease severity.Frontiers in plant science · 2024Article
- Article
- Measuring DNA modifications with the comet assay: a compendium of protocols.Nature protocols · 2023Review
- Article
- REPercussions: how geminiviruses recruit host factors for replication.Frontiers in microbiology · 2023Review
- Plant DNA polymerases α and δ mediate replication of geminiviruses.Nature communications · 2021Article
- DNA Geminivirus Infection Induces an Imprinted E3 Ligase Gene to Epigenetically Activate Viral Gene Transcription.The Plant cell · 2020Article
- Localization of Tobacco Yellow Dwarf Virus Replication Using the In Plant Activation (INPACT) Expression Platform.Viruses · 2020Article
- Comprehensive molecular insights into the stress response dynamics of rice (Journal of biosciences · 2020Article
- Multifunctional roles of geminivirus encoded replication initiator protein.Virusdisease · 2019Article
- Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots.Plant methods · 2018Article
- Two Novel DNAs That Enhance Symptoms and Overcome CMD2 Resistance to Cassava Mosaic Disease.Journal of virology · 2016Article
- Early function of the Abutilon mosaic virus AC2 gene as a replication brake.Journal of virology · 2015Article
- Rad54 is not essential for any geminiviral replication mode in planta.Plant molecular biology · 2015Article
- The genomes of many yam species contain transcriptionally active endogenous geminiviral sequences that may be functionally expressed.Virus evolution · 2015Article
- Geminiviruses: masters at redirecting and reprogramming plant processes.Nature reviews. Microbiology · 2013Review
- How can plant DNA viruses evade siRNA-directed DNA methylation and silencing?International journal of molecular sciences · 2013Review
- Regulating DNA replication in plants.Cold Spring Harbor perspectives in biology · 2012Review
- Expression of geminiviral AC2 RNA silencing suppressor changes sugar and jasmonate responsive gene expression in transgenic tobacco plants.BMC plant biology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The geminivirus Tomato golden mosaic virus (TGMV) replicates in differentiated plant cells using host DNA synthesis machinery. We used 5-bromo-2-deoxyuridine (BrdU) incorporation to examine DNA synthesis directly in infected Nicotiana benthamiana plants to determine if viral reprogramming of host replication controls had an impact on host DNA replication. Immunoblot analysis revealed that up to 17-fold more BrdU was incorporated into chromosomal DNA of TGMV-infected versus mock-infected, similarly treated healthy leaves. Colocalization studies of viral DNA and BrdU demonstrated that BrdU incorporation was specific to infected cells and was associated with both host and viral DNA. TGMV and host DNA synthesis were inhibited differentially by aphidicolin but were equally sensitive to hydroxyurea. Short BrdU labeling times resulted in some infected cells showing punctate foci associated with host DNA. Longer periods showed BrdU label uniformly throughout host DNA, some of which showed condensed chromatin, only in infected nuclei. By contrast, BrdU associated with viral DNA was centralized and showed uniform, compartmentalized labeling. Our results demonstrate that chromosomal DNA is replicated in TGMV-infected cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.