ArticleThe Plant cell2002
A high-throughput Arabidopsis reverse genetics system.
Article in The Plant cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 479 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
479 citing papers in PubMed, 961 citations in OpenAlex.
- Two proteins co-expressed with photosynthetic regulators affect photosynthetic dynamics linked to changes in thylakoid pigment content and composition.Plant biology (Stuttgart, Germany) · 2026Article
- The plant Golden Gate toolkit: compatibility, interoperability, and harmonized assembly in plant synthetic biology.Plant methods · 2026Review
- PEK14: A Kinesin-4 Necessary for Male-Derived Fertility in Arabidopsis thaliana.Cytoskeleton (Hoboken, N.J.) · 2026Article
- TONSL suppresses polymerase theta-dependent tandem duplications through chromatin-guided repair.Nature communications · 2026Article
- Phytosulfokine signalling blocks mycotoxin toxicity in Arabidopsis and mediates suppression of cell death activated by bacterial microbe-associated molecular patterns.The New phytologist · 2026Article
- Transcription factors instruct DNA methylation patterns in plant reproductive tissues.Nature cell biology · 2025Article
- Alterations in the proteomic profiles of Arabidopsis roots triggered by sodium chloride stress are exacerbated by impaired function of SYNAPTOTAGMIN 1.BMC plant biology · 2025Article
- A common pathway controls cell size in the sepal and leaf epidermis leading to a nonrandom pattern of giant cells.PLoS biology · 2025Article
- Homeodomain transcription factor regulation of leaf serrations in Arabidopsis thaliana.Journal of experimental botany · 2025Article
- Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.Journal of experimental botany · 2025Article
- Light and Shadows: Insights from Large-Scale Visual Screens for Arabidopsis Leaf Morphology Mutants.International journal of molecular sciences · 2025Review
- T-DNAreader: fast and precise identification of T-DNA insertion sites in plant genomes using RNA sequencing data.Genome biology · 2025Article
- A point mutation in the photosystem II protein PsbW disrupts thylakoid organization and alters starch granule formation.Plant physiology · 2025Article
- Analysis of xyloglucan metabolism mutants highlights the prominent role of xylose cleavage in seed dormancy.The Plant journal : for cell and molecular biology · 2025Article
- A data-integrative modeling approach accurately characterizes the effects of mutations on Arabidopsis lipid metabolism.Plant physiology · 2025Article
- The Arabidopsis KASH protein SINE3 is involved in male and female gametogenesis.Plant reproduction · 2024Article
- CO-EXPRESSED WITH PSI ASSEMBLY1 (CEPA1) is a photosystem I assembly factor in Arabidopsis.The Plant cell · 2024Article
- Article
- Phytochrome-dependent responsiveness to root-derived cytokinins enables coordinated elongation responses to combined light and nitrate cues.Nature communications · 2024Article
- Overexpression of RPOTmp Being Targeted to Either Mitochondria or Chloroplasts inInternational journal of molecular sciences · 2024Article
419 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymmetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from approximately 100000 transformed lines. A total of 85108 TAIL-PCR products from 52964 T-DNA lines were sequenced and compared with the Arabidopsis genome to determine the positions of T-DNAs in each line. Predicted T-DNA insertion sites, when mapped, showed a bias against predicted coding sequences. Predicted insertion mutations in genes of interest can be identified using Arabidopsis Gene Index name searches or by BLAST (Basic Local Alignment Search Tool) search. Insertions can be confirmed by simple PCR assays on individual lines. Predicted insertions were confirmed in 257 of 340 lines tested (76%). This resource has been named SAIL (Syngenta Arabidopsis Insertion Library) and is available to the scientific community at www.tmri.org.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.