ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2002
Regulation of the membrane estrogen receptor-alpha: role of cell density, serum, cell passage number, and estradiol.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 47 citations in OpenAlex.
- A 3D-Printed Vascular-Like Perfusion Tumor-on-a-Chip Recapitulates High-Grade Breast Cancer With in Vivo-Relevant Drug Resistance.Advanced healthcare materials · 2026Article
- Delineating the Effects of Passaging and Exposure in a Longitudinal Study of Arsenic-Induced Squamous Cell Carcinoma in a HaCaT Cell Line Model.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Multifunctionalized biocatalytic P22 nanoreactor for combinatory treatment of ER+ breast cancer.Journal of nanobiotechnology · 2018Article
- 17β-estradiol regulates the expression of apolipoprotein M through estrogen receptor α-specific binding motif in its promoter.Lipids in health and disease · 2017Article
- Multi-well plate immunoassays for measuring signaling protein activations/deactivations and membrane vs. intracellular receptor levels.Methods in molecular biology (Clifton, N.J.) · 2014Article
- Membrane glucocorticoid receptor activation induces proteomic changes aligning with classical glucocorticoid effects.Molecular & cellular proteomics : MCP · 2013Article
- Mixtures of xenoestrogens disrupt estradiol-induced non-genomic signaling and downstream functions in pituitary cells.Environmental health : a global access science source · 2013Article
- Bisphenol S disrupts estradiol-induced nongenomic signaling in a rat pituitary cell line: effects on cell functions.Environmental health perspectives · 2013Article
- Increased expression of IL-1 receptors in response to IL-1β may produce more IL-6, IL-8, VEGF, and PGE₂ in senescent synovial cells induced in vitro than in presenescent cells.Rheumatology international · 2012Article
- Article
- Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2C receptors.BMC neuroscience · 2012Article
- Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells.Environmental health : a global access science source · 2010Article
- Estrogens of multiple classes and their role in mental health disease mechanisms.International journal of women's health · 2010Article
- Nongenomic signaling pathways of estrogen toxicity.Toxicological sciences : an official journal of the Society of Toxicology · 2010Review
- Tamoxifen-poly(ethylene glycol)-thiol gold nanoparticle conjugates: enhanced potency and selective delivery for breast cancer treatment.Bioconjugate chemistry · 2009Article
- Nongenomic actions of low concentration estrogens and xenoestrogens on multiple tissues.Molecular and cellular endocrinology · 2007Review
- Estradiol effects on the dopamine transporter - protein levels, subcellular location, and function.Journal of molecular signaling · 2006Article
- Membrane estrogen receptor-alpha levels predict estrogen-induced ERK1/2 activation in MCF-7 cells.Breast cancer research : BCR · 2005Article
- Membrane estrogen receptor-alpha levels in MCF-7 breast cancer cells predict cAMP and proliferation responses.Breast cancer research : BCR · 2005Article
- Proteins of multiple classes may participate in nongenomic steroid actions.Experimental biology and medicine (Maywood, N.J.) · 2003Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We used modified immunocytochemical conditions to quantify a membrane form of estrogen receptor-alpha (mERalpha) in a rat pituitary tumor cell line, GH3/B6/F10. We studied the regulation of mERalpha vs. levels of intracellular ERalpha (iERalpha) using our 96-well plate immunoassay. The anti-ERalpha antibody C542 was used to label the ERalpha (via conjugated alkaline phosphatase) in fixed permeabilized (for iERalpha) vs. nonpermeabilized cells (for mERalpha). Expression of mERalpha was highest at low cell densities (<1000 cells/well) and decreased significantly at densities where cellular processes touched, whereas the more abundant iERalpha increased with increasing cell density over the same range. Serum starvation for 48 h caused increases in mERalpha, whereas iERalpha levels showed no significant changes. A large decline in mERalpha and iERalpha levels with cell passage number was observed. Minutes after nM 17beta-estradiol (E2) treatment, a portion of the cells rounded up and detached from the culture plate, whereas nM cholesterol had no such effect. Although E2 treatment did not change mERalpha levels, the antigen was reorganized from a fine particulate to aggregation into asymmetric large granules of staining. That common culturing conditions favor down-regulation of mERalpha may explain the relatively few reports of this protein in other experimental systems.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.