ArticleNucleic acids research2002
Sequence tagged microsatellite profiling (STMP): improved isolation of DNA sequence flanking target SSRs.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 20 citations in OpenAlex.
- New tools to screen wild peanut species for aflatoxin accumulation and genetic fingerprinting.BMC plant biology · 2018Article
- Microsatellites reveal genetic diversity in Rotylenchulus reniformis populations.Journal of nematology · 2009Article
- Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- Genetic and in silico comparative mapping of the polyphenol oxidase gene in bread wheat (Triticum aestivum L.).Functional & integrative genomics · 2005Article
- Molecular markers from the transcribed/expressed region of the genome in higher plants.Functional & integrative genomics · 2004Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sequence tagged microsatellite profiling (STMP) enables the rapid development of large numbers of co-dominant DNA markers, known as sequence tagged microsatellites (STMs). Each STM is amplified by PCR using a single primer specific to the conserved DNA sequence flanking the microsatellite repeat in combination with a universal primer that anchors to the 5'-ends of the microsatellites. It is also possible to convert STMs into conventional microsatellite, or simple sequence repeat (SSR), markers that are amplified using a pair of primers flanking the repeat sequence. Here, we describe a modification of the STMP procedure to significantly improve the capacity to convert STMs into conventional SSRs and, therefore, facilitate the development of highly specific DNA markers for purposes such as marker-assisted breeding. The usefulness of this technique was demonstrated in bread wheat.
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