ArticleNucleic acids research2002
Proteomic analysis of human metaphase chromosomes reveals topoisomerase II alpha as an Aurora B substrate.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed.
- Mitotic chromosomes.Seminars in cell & developmental biology · 2021Review
- Proteome Analysis of Condensed Barley Mitotic Chromosomes.Frontiers in plant science · 2021Article
- α-Fodrin is required for the organization of functional microtubules during mitosis.Cell cycle (Georgetown, Tex.) · 2019Article
- TOP2B: The First Thirty Years.International journal of molecular sciences · 2018Review
- Characterization of Aurora A and Its Impact on the Effect of Cisplatin-Based Chemotherapy in Patients with Non-Small Cell Lung Cancer.Translational oncology · 2017Article
- Quantitative Proteomic Analysis of Replicative and Nonreplicative Forms Reveals Important Insights into Chromatin Biology of Trypanosoma cruzi.Molecular & cellular proteomics : MCP · 2017Article
- The C-terminal 20 Amino Acids of Drosophila Topoisomerase 2 Are Required for Binding to a BRCA1 C Terminus (BRCT) Domain-containing Protein, Mus101, and Fidelity of DNA Segregation.The Journal of biological chemistry · 2016Article
- Article
- TOPBP1 recruits TOP2A to ultra-fine anaphase bridges to aid in their resolution.Nature communications · 2015Article
- Proteomics in epigenetics: new perspectives for cancer research.Briefings in functional genomics · 2013Review
- Mass spectrometry-based proteomics for the analysis of chromatin structure and dynamics.International journal of molecular sciences · 2013Article
- Lasonolide A, a potent and reversible inducer of chromosome condensation.Cell cycle (Georgetown, Tex.) · 2012Article
- Building mitotic chromosomes.Current opinion in cell biology · 2011Review
- Multiclassifier proteomics to define complexes yields new chromosomal proteins.Developmental cell · 2010Article
- Article
- Nuclear matrix proteome analysis of Drosophila melanogaster.Molecular & cellular proteomics : MCP · 2010Article
- Identification of Myb-binding protein 1A (MYBBP1A) as a novel substrate for aurora B kinase.The Journal of biological chemistry · 2010Article
- The middle region of an HP1-binding protein, HP1-BP74, associates with linker DNA at the entry/exit site of nucleosomal DNA.The Journal of biological chemistry · 2010Article
- Gene expression changes are age-dependent and lobe-specific in the brown Norway rat model of prostatic hyperplasia.The Prostate · 2009Article
- DNA topoisomerase II and its growing repertoire of biological functions.Nature reviews. Cancer · 2009Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
The essential Aurora B kinase is a chromosomal passenger protein that is required for mitotic chromosome alignment and segregation. Aurora B function is dependent on the chromosome passenger, INCENP. INCENP, in turn, requires sister chromatid cohesion for its appropriate behaviour. Relatively few substrates have been identified for Aurora B, so that the precise role it plays in controlling mitosis remains to be elucidated. To identify potential novel mitotic substrates of Aurora B, extracted chromosomes were prepared from mitotically-arrested HeLa S3 cells and incubated with recombinant human Aurora B in the presence of radioactive ATP. Immunoblot analysis confirmed the HeLa scaffold fraction to be enriched for known chromosomal proteins including CENP-A, CENP-B, CENP-C, ScII and INCENP. Mass spectrometry of bands excised from one-dimensional polyacrylamide gels further defined the protein composition of the extracted chromosome fraction. Cloning, fluorescent tagging and expression in HeLa cells of the putative GTP-binding protein NGB/CRFG demonstrated it to be a novel mitotic chromosome protein, with a perichromosomal localisation. Identi fication of the protein bands corresponding to those phosphorylated by Aurora B revealed topoisomerase II alpha (topo IIalpha) as a potential Aurora B substrate. Purified recombinant human topo IIalpha was phosphorylated by Aurora B in vitro, confirming this proteomic approach as a valid method for the initial definition of candidate substrates of key mitotic kinases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.