ArticleNucleic acids research2002
Functional domains involved in the interaction between Orc1 and transcriptional repressor AlF-C that bind to an origin/promoter of the rat aldolase B gene.
Article in Nucleic acids research, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 22 citations in OpenAlex.
- The plant cell cycle: Pre-Replication complex formation and controls.Genetics and molecular biology · 2017Article
- Epstein-Barr nuclear antigen 1 (EBNA1)-dependent recruitment of origin recognition complex (Orc) on oriP of Epstein-Barr virus with purified proteins: stimulation by Cdc6 through its direct interaction with EBNA1.The Journal of biological chemistry · 2012Article
- Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family.American journal of physiology. Endocrinology and metabolism · 2010Article
- DNA replication and the GINS complex: localization on extended chromatin fibers.Epigenetics & chromatin · 2009Article
- Transcription initiation activity sets replication origin efficiency in mammalian cells.PLoS genetics · 2009Article
- Arabidopsis ORC1 is a PHD-containing H3K4me3 effector that regulates transcription.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- RNA-dependent recruitment of the origin recognition complex.The EMBO journal · 2008Article
- Heterogeneous nuclear ribonucleoprotein A/B and G inhibits the transcription of gonadotropin-releasing-hormone 1.Molecular and cellular neurosciences · 2008Article
- Structure and allele-specific expression variation of novel alpha/beta hydrolase fold proteins in gentian plants.Molecular genetics and genomics : MGG · 2007Article
- Binding of AlF-C, an Orc1-binding transcriptional regulator, enhances replicator activity of the rat aldolase B origin.Molecular and cellular biology · 2006Article
- ORC binding to TRF2 stimulates OriP replication.EMBO reports · 2006Article
- The highly conserved tRNAHis guanylyltransferase Thg1p interacts with the origin recognition complex and is required for the G2/M phase transition in the yeast Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
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4 authors at 1 institution in 1 country.
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Abstract
The promoter of the rat aldolase B (AldB) gene functions in vivo as an origin of DNA replication in the cells in which transcription of the gene is repressed. Previously, we identified two closely related DNA-binding proteins, AlF-C1 and AlF-C2, which repressed the AldB gene promoter. We also reported that the binding site of these proteins, site C, is one of the required DNA elements of the AldB gene origin/promoter for autonomously replicating activity in transfected cells. In the present study, we show that AlF-C1 and AlF-C2 bind directly to Orc1, a subunit of the origin recognition complex (ORC). Deletion analyses revealed a functional domain in AlF-C2 for binding to Orc1, which is located separately from the DNA-binding domain. In addition, we found a novel protein-interacting domain in Orc1 required for the binding of AlF-C2, which was conserved in human, mouse and Chinese hamster, but not in Drosophila, frog and yeast. Thus, it is assumed that in mammalian cells, sequence- specific DNA-binding proteins are involved in recruiting ORC to regulate replication initiation and/or transcription repression.
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