ArticleProceedings of the National Academy of Sciences of the United States of America2002
Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: application to single virus-like particle entry into a cell.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 103 citations in OpenAlex.
- Development of scanning ion-conductance microscopy based tip-enhanced Raman spectroscopy in liquid environments.Nanotechnology · 2026Review
- A Correlative SICM-OPM Platform for Surface and Volumetric Imaging in Live Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A Look inside a Flexible Open-Source Scanning Electrochemical Probe Microscope.ACS electrochemistry · 2026Article
- Review
- Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts.Communications biology · 2023Article
- Diversity of cells and signals in the cardiovascular system.The Journal of physiology · 2023Article
- Scanning Ion Conductance Microscopy.Chemical reviews · 2021Review
- Correlative 3D microscopy of single cells using super-resolution and scanning ion-conductance microscopy.Nature communications · 2021Article
- Studying signal compartmentation in adult cardiomyocytes.Biochemical Society transactions · 2020Review
- Variations in Plasma Membrane Topography Can Explain Heterogenous Diffusion Coefficients Obtained by Fluorescence Correlation Spectroscopy.Frontiers in cell and developmental biology · 2020Article
- Correlative SICM-FCM reveals changes in morphology and kinetics of endocytic pits induced by disease-associated mutations in dynamin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Article
- Imaging, Tracking and Computational Analyses of Virus Entry and Egress with the Cytoskeleton.Viruses · 2018Review
- Angular Approach Scanning Ion Conductance Microscopy.Biophysical journal · 2016Article
- The application of virus-like particles as vaccines and biological vehicles.Applied microbiology and biotechnology · 2015Review
- Article
- Imaging the cell surface and its organization down to the level of single molecules.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Review
- Scanning ion conductance microscopy for studying biological samples.Sensors (Basel, Switzerland) · 2012Review
- Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Scanning ion conductance microscopy: a nanotechnology for biological studies in live cells.Frontiers in physiology · 2012Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a method for simultaneous recording of high-resolution topography and cell surface fluorescence in a single scan which we call scanning surface confocal microscopy. The resolution of the system allows imaging of individual fluorescent particles in the nanometer range on fixed or live cells. We used this technique to record the interaction of single virus-like particles with the cell surface and demonstrated that single particles sink into the membrane in invaginations reminiscent of caveolae or pinocytic vesicles. This method provides a technique for elucidating the interaction of individual viruses and other nanoparticles, such as gene therapy vectors, with target cells. Furthermore, this technique should find widespread application for studying the relationship of fluorescently tagged molecules with components of the cell plasma membrane.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.