ArticleThe Journal of cell biology2002
CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA.
Article in The Journal of cell biology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 159 papers.
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Who cites it
159 citing papers in PubMed, 300 citations in OpenAlex.
- α-Satellite RNA maintains centromeric cohesion through R-loops.Molecular cell · 2026Article
- Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation.Nucleic acids research · 2026Article
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Article
- Sustainable integrative cell biology: CENP-C is guilty by association.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Genomic and epigenomic maps of mouse centromeres and pericentromeres.Nature communications · 2025Article
- Revisiting the question: When is a centromere not a kinetochore?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- The dynamic centromere.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Review
- Rapid assembly of functional modules for generating human artificial chromosome constructs compatible with epigenetic centromere seeding.bioRxiv : the preprint server for biology · 2025Article
- Non-canonical DNA in human and other ape telomere-to-telomere genomes.Nucleic acids research · 2025Article
- Non-canonical DNA in human and other ape telomere-to-telomere genomes.bioRxiv : the preprint server for biology · 2025Article
- Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA.Nucleic acids research · 2025Article
- The impact of mosaic loss of the Y chromosome (mLOY) in men of advanced age.Biogerontology · 2024Review
- Loss of the Y Chromosome: A Review of Molecular Mechanisms, Age Inference, and Implications for Men's Health.International journal of molecular sciences · 2024Review
- DNA strand breaks at centromeres: Friend or foe?Seminars in cell & developmental biology · 2024Review
- CENP-A and CENP-B collaborate to create an open centromeric chromatin state.Nature communications · 2023Article
- Variation in the CENP-A sequence association landscape across diverse inbred mouse strains.Cell reports · 2023Article
- p97/VCP drives turnover of SUMOylated centromeric CCAN proteins and CENP-A.Molecular biology of the cell · 2023Article
- CENP-I directly targets centromeric DNA to support CENP-A deposition and centromere maintenance.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
99 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromere protein (CENP) B boxes, recognition sequences of CENP-B, appear at regular intervals in human centromeric alpha-satellite DNA (alphoid DNA). In this study, to determine whether information carried by the primary sequence of alphoid DNA is involved in assembly of functional human centromeres, we created four kinds of synthetic repetitive sequences: modified alphoid DNA with point mutations in all CENP-B boxes, resulting in loss of all CENP-B binding activity; unmodified alphoid DNA containing functional CENP-B boxes; and nonalphoid repetitive DNA sequences with or without functional CENP-B boxes. These four synthetic repetitive DNAs were introduced into cultured human cells (HT1080), and de novo centromere assembly was assessed using the mammalian artificial chromosome (MAC) formation assay. We found that both the CENP-B box and the alphoid DNA sequence are required for de novo MAC formation and assembly of functional centromere components such as CENP-A, CENP-C, and CENP-E. Using the chromatin immunoprecipitation assay, we found that direct assembly of CENP-A and CENP-B in cells with synthetic alphoid DNA required functional CENP-B boxes. To the best of our knowledge, this is the first reported evidence of a functional molecular link between a centromere-specific DNA sequence and centromeric chromatin assembly in humans.
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