ArticleThe Journal of physiology2002
Functional regulation of xanthine oxidoreductase expression and localization in the mouse mammary gland: evidence of a role in lipid secretion.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 133 citations in OpenAlex.
- Comparative proteomic analysis of human milk fat globules and paired membranes and mouse milk fat globules identifies core cellular systems contributing to mammary lipid trafficking and secretion.Frontiers in molecular biosciences · 2023Article
- The double faced role of xanthine oxidoreductase in cancer.Acta pharmacologica Sinica · 2022Review
- Perilipin-2 promotes lipid droplet-plasma membrane interactions that facilitate apocrine lipid secretion in secretory epithelial cells of the mouse mammary gland.Frontiers in cell and developmental biology · 2022Article
- Improving Human Health with Milk Fat Globule Membrane, Lactic Acid Bacteria, and Bifidobacteria.Microorganisms · 2021Review
- Milk somatic cell derived transcriptome analysis identifies regulatory genes and pathways during lactation in Indian Sahiwal cattle (Bos indicus).Molecular biology reports · 2020Article
- Xanthine oxidase-lactoperoxidase system and innate immunity: Biochemical actions and physiological roles.Redox biology · 2020Review
- TDP-43 facilitates milk lipid secretion by post-transcriptional regulation of Btn1a1 and Xdh.Nature communications · 2020Article
- Symposium review: Intravital imaging of the lactating mammary gland in live mice reveals novel aspects of milk-lipid secretion.Journal of dairy science · 2019Article
- Milk lipid regulation at the maternal-offspring interface.Seminars in cell & developmental biology · 2018Review
- Th-POK regulates mammary gland lactation through mTOR-SREBP pathway.PLoS genetics · 2018Article
- Ultrastructural and immunocytochemical evidence for the reorganisation of the milk fat globule membrane after secretion.Cell and tissue research · 2017Article
- Xanthine oxidoreductase mediates membrane docking of milk-fat droplets but is not essential for apocrine lipid secretion.The Journal of physiology · 2016Article
- Xanthine oxidoreductase in cancer: more than a differentiation marker.Cancer medicine · 2016Review
- Immunocytochemical Evidence for Golgi Vesicle Involvement in Milk Fat Globule Secretion.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2015Article
- Proteomics-driven analysis of ovine whey colostrum.PloS one · 2015Article
- Cholesterol transport and regulation in the mammary gland.Journal of mammary gland biology and neoplasia · 2014Review
- Structural and functional insights into the catalytic inactivity of the major fraction of buffalo milk xanthine oxidoreductase.PloS one · 2014Article
- A test of current models for the mechanism of milk-lipid droplet secretion.Traffic (Copenhagen, Denmark) · 2013Article
- Milk lipid secretion: recent biomolecular aspects.Biomolecular concepts · 2012Article
- Mutations associated with functional disorder of xanthine oxidoreductase and hereditary xanthinuria in humans.International journal of molecular sciences · 2012Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Xanthine oxidoreductase (XOR), a key enzyme of purine metabolism, has been implicated in the secretion of the milk fat droplet in lactating mammary epithelial cells, possibly through structural interactions with other milk fat globule proteins including butyrophilin (Btn) and adipophilin (ADPH). To help determine the mechanism by which XOR is regulated, we examined the expression and localization of XOR in the non-secretory states of late pregnancy and induced involution compared with the state of active secretion. XOR mRNA levels started to increase at mid-pregnancy, turned sharply upwards at the onset of lactation and decreased rapidly with forced involution, indicating transcriptional control of the enzyme level by differentiation and secretory function. During pregnancy and involution the enzyme was diffusely distributed in the cytoplasm, but moved rapidly to the apical membrane of the cells when secretion was activated, where it colocalized with both Btn and ADPH, similar to the situation in the milk fat globule itself. Size-exclusion chromatography of solubilized milk fat globule membrane proteins showed that XOR formed a sulphydryl-bond-dependent complex with Btn and ADPH in the milk fat globule membrane. XOR returned to a diffuse cytoplasmic localization shortly after induced involution, while Btn remained localized to the apical membrane, suggesting that localization of XOR is not dependent on the presence of Btn in the apical membrane. Our findings indicate that the expression and membrane association of XOR in the mammary gland are tightly regulated by secretory activity, and suggest that the apical membrane association of XOR regulates the coupling of lipid droplets to the apical plasma membrane during milk lipid secretion.
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