ArticleThe Journal of physiology2002
Flufenamic acid blocks depolarizing afterpotentials and phasic firing in rat supraoptic neurones.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 73 citations in OpenAlex.
- Muscarinic acetylcholine receptor activated effectors in principal neurons of the rat basolateral amygdala.Frontiers in cellular neuroscience · 2025Article
- Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats.International journal of molecular sciences · 2022Article
- Phasic spiking in vasopressin neurons: How and Why.Journal of neuroendocrinology · 2021Review
- Electrophysiological properties of identified oxytocin and vasopressin neurones.Journal of neuroendocrinology · 2019Review
- Oxytocin Neurones: Intrinsic Mechanisms Governing the Regularity of Spiking Activity.Journal of neuroendocrinology · 2016Article
- Gap Junctions Contribute to the Regulation of Walking-Like Activity in the Adult Mudpuppy (Necturus Maculatus).PloS one · 2016Article
- Transient and sustained afterdepolarizations in accessory olfactory bulb mitral cells are mediated by distinct mechanisms that are differentially regulated by neuromodulators.Frontiers in cellular neuroscience · 2014Article
- Enhanced NMDA receptor-mediated intracellular calcium signaling in magnocellular neurosecretory neurons in heart failure rats.American journal of physiology. Regulatory, integrative and comparative physiology · 2013Article
- Dendritic peptide release mediates interpopulation crosstalk between neurosecretory and preautonomic networks.Neuron · 2013Article
- Calcium-activated sustained firing responses distinguish accessory from main olfactory bulb mitral cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Phasic firing in vasopressin cells: understanding its functional significance through computational models.PLoS computational biology · 2012Article
- Dopamine-induced oscillations of the pyloric pacemaker neuron rely on release of calcium from intracellular stores.Journal of neurophysiology · 2011Article
- Flufenamic acid decreases neuronal excitability through modulation of voltage-gated sodium channel gating.The Journal of physiology · 2010Article
- Flufenamic acid modulates multiple currents in gonadotropin-releasing hormone neurons.Brain research · 2010Article
- Rebound discharge in deep cerebellar nuclear neurons in vitro.Cerebellum (London, England) · 2010Review
- Performance, properties and plasticity of identified oxytocin and vasopressin neurones in vitro.Journal of neuroendocrinology · 2010Review
- Muscarinic receptor activation modulates the excitability of hilar mossy cells through the induction of an afterdepolarization.Brain research · 2010Article
- Glutamatergic inputs contribute to phasic activity in vasopressin neurons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Group I mGluR activation enhances Ca(2+)-dependent nonselective cation currents and rhythmic bursting in main olfactory bulb external tufted cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Excitability of paraventricular nucleus neurones that project to the rostral ventrolateral medulla is regulated by small-conductance Ca2+-activated K+ channels.The Journal of physiology · 2009Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Depolarizing afterpotentials (DAPs) that follow action potentials in magnocellular neurosecretory cells (MNCs) are thought to underlie the generation of phasic firing, a pattern that optimizes vasopressin release from the neurohypophysis. Previous work has suggested that the DAP may result from the Ca(2+)-dependent reduction of a resting K(+) conductance. Here we examined the effects of flufenamic acid (FFA), a blocker of Ca(2+)-dependent non-selective cation (CAN) channels, on DAPs and phasic firing using intracellular recordings from supraoptic MNCs in superfused explants of rat hypothalamus. Application of FFA, but not solvent (0.1 % DMSO), reversibly inhibited (IC(50) = 13.8 microM; R = 0.97) DAPs and phasic firing with a similar time course, but had no significant effects (P > 0.05) on membrane potential, spike threshold and input resistance, nor on the frequency and amplitude of spontaneous synaptic potentials. Moreover, FFA did not affect (P > 0.05) the amplitude, duration, undershoot, or frequency-dependent broadening of action potentials elicited during the spike trains used to evoke DAPs. These findings suggest that FFA inhibits the DAP by directly blocking the channels responsible for its production, rather than by interfering with Ca(2+) influx. They also support a role for DAPs in the generation of phasic firing in MNCs. Finally, the absence of a depolarization and increased membrane resistance upon application of FFA suggests that the DAP in MNCs may not be due to the inhibition of resting K(+) current, but to the activation of CAN channels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.