ArticleThe Journal of physiology2002
Single-cell epithelial defects close rapidly by an actinomyosin purse string mechanism with functional tight junctions.
Article in The Journal of physiology, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 78 citations in OpenAlex.
- Epithelial restitution in 3D - Revealing biomechanical and physiochemical dynamics in intestinal organoids via fs laser nanosurgery.iScience · 2023Article
- Cellular mechanisms of wound closure under cyclic stretching.Biophysical journal · 2023Article
- Unraveling the intestinal epithelial barrier in cyanotoxin microcystin-treated Caco-2 cell monolayers.Annals of the New York Academy of Sciences · 2022Article
- Synaptopodin stress fiber and contractomere at the epithelial junction.The Journal of cell biology · 2022Article
- NF-κB Inducing Kinase Regulates Intestinal Immunity and Homeostasis.Frontiers in immunology · 2022Review
- Article
- Diarrheal Mechanisms and the Role of Intestinal Barrier Dysfunction in Campylobacter Infections.Current topics in microbiology and immunology · 2021Article
- Contribution ofFrontiers in microbiology · 2020Article
- Autoregulatory "Multitasking" at Endothelial Cell Junctions by Junction-Associated Intermittent Lamellipodia Controls Barrier Properties.Frontiers in physiology · 2020Review
- Effects ofPharmaceuticals (Basel, Switzerland) · 2018Article
- The role of Rho kinase (Rock) in re-epithelialization of adult zebrafish skin wounds.Small GTPases · 2018Article
- Single-Cell Defects Cause a Long-Range Mechanical Response in a Confluent Epithelial Cell Layer.Biophysical journal · 2017Article
- Physical Model for Stabilization and Repair of Trans-endothelial Apertures.Biophysical journal · 2017Article
- Mechanics of epithelial tissues during gap closure.Current opinion in cell biology · 2016Review
- Life and death at the mucosal-luminal interface: New perspectives on human intestinal ischemia-reperfusion.World journal of gastroenterology · 2016Review
- Gap geometry dictates epithelial closure efficiency.Nature communications · 2015Article
- Potentiometric-scanning ion conductance microscopy for measurement at tight junctions.Tissue barriers · 2013Review
- Myosin transcellular networks regulate epithelial apical geometry.Cell cycle (Georgetown, Tex.) · 2013Article
- Scanning ion conductance microscopy measurement of paracellular channel conductance in tight junctions.Analytical chemistry · 2013Article
- Cell crawling mediates collective cell migration to close undamaged epithelial gaps.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Restitution of single-cell defects, a frequent event in epithelia with high turnover, is poorly understood. Morphological and functional changes were recorded, using intravital time-lapse video microscopy, confocal fluorescence microscopy, and conductance scanning techniques. After artificial single-cell loss from an HT-29/B6 colonic cell monolayer, the basal ends of adjacent cells extended. Concurrently, the local conductive leak associated with the defect sealed with an exponential time course (from 0.48 +/- 0.05 microS 2 min post lesion to 0.17 +/- 0.02 microS 8 min post lesion, n = 17). Between 3 and 10 min post lesion, a band of actin arose around the gap, which colocalized with a ring of ZO-1 and occludin. Hence, tight junction proteins bound to the actin band facing the gap, and competent tight junctions assembled in the adjoining cell membranes. Closure and sealing were inhibited when actin polymerization was blocked by cytochalasin D, delayed following decrease of myosin-ATPase activity by butanedione monoxime, and blocked after myosin light chain kinase inhibition by ML-7. The Rho-associated protein kinase inhibitor Y-27632 did not affect restitution. After loosening of intercellular contacts in low Ca(2+) Ringer solution, the time course of restitution was not significantly altered. Albeit epithelial conductivity was 12-fold higher in low Ca(2+) Ringer solution than in controls, under both conditions the repaired epithelium assumed the same conductivity as distant intact epithelium. In conclusion, epithelial restitution of single-cell defects comprises rapid closure by an actinomyosin 'purse-string' mechanism and simultaneous formation of a functional barrier from tight junction proteins also associated with the purse string.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.