ArticleThe EMBO journal2002
Lack of mitochondrial citrate synthase discloses a new meiotic checkpoint in a strict aerobe.
Article in The EMBO journal, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Protein Profiling ofInternational journal of molecular sciences · 2023Article
- Integrative Analysis of Transcriptomic and Proteomic Changes Related to Cytoplasmic Male Sterility in Spring Stem Mustard (International journal of molecular sciences · 2022Article
- Transcriptome Profiling Reveals Molecular Changes during Flower Development between Male Sterile and Fertile Chinese Cabbage (Life (Basel, Switzerland) · 2021Article
- Viral growth factor- and STAT3 signaling-dependent elevation of the TCA cycle intermediate levels during vaccinia virus infection.PLoS pathogens · 2021Article
- Dynamic Regulation of Peroxisomes and Mitochondria during Fungal Development.Journal of fungi (Basel, Switzerland) · 2020Review
- Article
- NEK10 interactome and depletion reveal new roles in mitochondria.Proteome science · 2020Article
- Distinct Contributions of the Peroxisome-Mitochondria Fission Machinery During Sexual Development of the FungusFrontiers in microbiology · 2020Article
- Metabolic and developmental effects resulting from deletion of the citA gene encoding citrate synthase in Aspergillus nidulans.Eukaryotic cell · 2010Article
- A two-step protocol for efficient deletion of genes in the filamentous ascomycete Podospora anserina.Current genetics · 2005Article
- Altering a gene involved in nuclear distribution increases the repeat-induced point mutation process in the fungus Podospora anserina.Genetics · 2004Article
- Article
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Authors and funding
5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Mitochondrial citrate synthase (mCS) is the initial enzyme of the tricarboxylic acid (TCA) cycle. Despite the key position of this protein in respiratory metabolism, very few studies have addressed the question of the effects of the absence of mCS in development. Here we report on the characterization of 15 point mutations and a complete deletion of the cit1 gene, which encodes mCS in the filamentous fungus Podospora anserina. This gene was identified genetically through a systematic search for suppressors of the metabolic defect of the peroxisomal pex2 mutants. The cit1 mutant strains exhibit no visible vegetative defects. However, they display an unexpected developmental phenotype: in homozygous crosses, cit1 mutations impair meiosis progression beyond the diffuse stage, a key stage of meiotic prophase. Enzyme assays, immunofluorescence and western blotting experiments show that the presence of the mCS protein is more important for completion of meiosis than its well-known enzyme activity. Combined with observations made in budding yeast, our data suggest that there is a general metabolic checkpoint at the diffuse stage in eukaryotes.
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