ArticleEukaryotic cell2002
Functional diversity of silencers in budding yeasts.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 25 citations in OpenAlex.
- Intergeneric chromosomal transfer in yeast results in improved phenotypes and widespread transcriptional responses.Nature communications · 2026Article
- Context-dependent function of the transcriptional regulator Rap1 in gene silencing and activation inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- An Evolutionary Perspective on Yeast Mating-Type Switching.Genetics · 2017Review
- Linking replication stress with heterochromatin formation.Chromosoma · 2016Review
- Evolution and Functional Trajectory of Sir1 in Gene Silencing.Molecular and cellular biology · 2016Article
- Review
- Reinventing heterochromatin in budding yeasts: Sir2 and the origin recognition complex take center stage.Eukaryotic cell · 2011Review
- Tight protein-DNA interactions favor gene silencing.Genes & development · 2011Article
- Transcriptional silencing functions of the yeast protein Orc1/Sir3 subfunctionalized after gene duplication.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Ume6 is required for the MATa/MATalpha cellular identity and transcriptional silencing in Kluyveromyces lactis.Genetics · 2010Article
- Alpha3, a transposable element that promotes host sexual reproduction.Genes & development · 2010Article
- The Sir2-Sum1 complex represses transcription using both promoter-specific and long-range mechanisms to regulate cell identity and sexual cycle in the yeast Kluyveromyces lactis.PLoS genetics · 2009Article
- Cis-motifs upstream of the transcription and translation initiation sites are effectively revealed by their positional disequilibrium in eukaryote genomes using frequency distribution curves.BMC bioinformatics · 2006Article
- Evolution of the MAT locus and its Ho endonuclease in yeast species.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region.Nucleic acids research · 2003Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We studied the silencing of the cryptic mating-type loci HMLa and HMRa in the budding yeast Kluyveromyces lactis. A 102-bp minimal silencer fragment was defined that was both necessary and sufficient for silencing of HMLalpha. Mutagenesis of the silencer revealed three distinct regions (A, B, and C) that were important for silencing. Recombinant K. lactis ribosomal DNA enhancer binding protein 1 (Reb1p) could bind the silencer in vitro, and point mutations in the B box abolished both Reb1p binding and silencer function. Furthermore, strains carrying temperature-sensitive alleles of the REBI gene derepressed the transcription of the HMLalpha1 gene at the nonpermissive temperature. A functional silencer element from the K. lactis cryptic HMRa locus was also identified, which contained both Reb1p binding sites and A boxes, strongly suggesting a general role for these sequences in K lactis silencing. Our data indicate that different proteins bind to Kluyveromyces silencers than to Saccharomyces silencers. We suggest that the evolution of silencers is rapid in budding yeasts and discuss the similarities and differences between silencers in Saccharomyces and Kluyveromyces.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.