ArticleEukaryotic cell2002
Mga2p processing by hypoxia and unsaturated fatty acids in Saccharomyces cerevisiae: impact on LORE-dependent gene expression.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 51 citations in OpenAlex.
- Alterations to the yeast chromatin landscape during hypoxia.bioRxiv : the preprint server for biology · 2026Article
- Nrf1 acts as a highly-conserved determinon for maintaining robust redox homeostasis in the eco-evo-devo process of life histories.Cell stress · 2025Review
- The effect of hypoxia on the lipidome of recombinant Pichia pastoris.Microbial cell factories · 2017Article
- Three, two, one yeast fatty acid desaturases: regulation and function.World journal of microbiology & biotechnology · 2017Review
- Coordinate Regulation of Yeast Sterol Regulatory Element-binding Protein (SREBP) and Mga2 Transcription Factors.The Journal of biological chemistry · 2017Article
- Engineering Ashbya gossypii strains for de novo lipid production using industrial by-products.Microbial biotechnology · 2017Article
- Time-Course Analysis of Gene Expression During the Saccharomyces cerevisiae Hypoxic Response.G3 (Bethesda, Md.) · 2017Article
- Article
- The nuclear localization of SWI/SNF proteins is subjected to oxygen regulation.Cell & bioscience · 2012Article
- Host acyl coenzyme A binding protein regulates replication complex assembly and activity of a positive-strand RNA virus.Journal of virology · 2012Article
- Hypoxia elicits broad and systematic changes in protein subcellular localization.American journal of physiology. Cell physiology · 2011Article
- Heme biosynthesis and its regulation: towards understanding and improvement of heme biosynthesis in filamentous fungi.Applied microbiology and biotechnology · 2011Review
- Deletion of a subgroup of ribosome-related genes minimizes hypoxia-induced changes and confers hypoxia tolerance.Physiological genomics · 2011Article
- The Hog1 mitogen-activated protein kinase mediates a hypoxic response in Saccharomyces cerevisiae.Genetics · 2011Article
- Effects of different carbon sources on the growth, fatty acids production, and expression of three desaturase genes of Mortierella alpina ATCC 16266.Current microbiology · 2011Article
- Structural and functional insights into Saccharomyces cerevisiae Tpa1, a putative prolylhydroxylase influencing translation termination and transcription.The Journal of biological chemistry · 2010Article
- Harnessing gene expression to identify the genetic basis of drug resistance.Molecular systems biology · 2009Article
- A predictive model of the oxygen and heme regulatory network in yeast.PLoS computational biology · 2008Article
- Oxygen-dependent transcriptional regulator Hap1p limits glucose uptake by repressing the expression of the major glucose transporter gene RAG1 in Kluyveromyces lactis.Eukaryotic cell · 2008Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, OLE1 encodes a delta9 fatty acid desaturase, an enzyme that plays a critical role in maintaining the correct ratio of saturated to monounsaturated fatty acids in the cell membrane. Previous studies have demonstrated that (i) OLE1 expression is repressed by unsaturated fatty acids (UFAs) and induced by low oxygen tension, (ii) a component of this regulation is mediated through the same low oxygen response element (LORE) in the OLE1 promoter, and (iii) Mga2p is involved in LORE-dependent hypoxic induction of OLE1. We now report that LORE-CYC1 basal promoter-lacZ fusion reporter assays demonstrate that UFAs repress the reporter expression under hypoxic conditions in a dose-dependent manner via LORE. Electrophoretic mobility shift assays show that UFAs repress the hypoxia-induced complex formation with LORE. Studies with a construct encoding a truncated form of Mga2p support the hypothesis that both hypoxia and UFA signals affect the processing of Mga2p and the UFA repression of OLE1 hypoxic induction is mediated through Mga2p. Data from Western blot assays provide evidence that under normoxic conditions, Mga2p processing produces approximately equimolar levels of the membrane-bound and processed forms and is unaffected by UFAs. Hypoxic induction of OLE1, however, is associated with increased processing of the protein, resulting in an approximately fivefold increase in the soluble active form that is counteracted by exposure of the cells to unsaturated fatty acids. Data from this study suggest that the Mga2p-LORE interaction plays an important role in OLE1 expression under both normoxic and hypoxic conditions.
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