ArticleEukaryotic cell2002
Divergent subunit interactions among fungal mRNA 5'-capping machineries.
Article in Eukaryotic cell, 2002. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 22 citations in OpenAlex.
- Defenders of the Transcriptome: Guard Protein-Mediated mRNA Quality Control inInternational journal of molecular sciences · 2024Review
- A guard protein mediated quality control mechanism monitors 5'-capping of pre-mRNAs.Nucleic acids research · 2022Article
- A Saccharomyces cerevisiae model and screen to define the functional consequences of oncogenic histone missense mutations.G3 (Bethesda, Md.) · 2022Article
- Interplay of mRNA capping and transcription machineries.Bioscience reports · 2020Review
- Cap-binding complex (CBC).The Biochemical journal · 2014Review
- Structure of the Saccharomyces cerevisiae Cet1-Ceg1 mRNA capping apparatus.Structure (London, England : 1993) · 2010Article
- TFIIH and P-TEFb coordinate transcription with capping enzyme recruitment at specific genes in fission yeast.Molecular cell · 2009Article
- Cyclin-dependent kinase 9 (Cdk9) of fission yeast is activated by the CDK-activating kinase Csk1, overlaps functionally with the TFIIH-associated kinase Mcs6, and associates with the mRNA cap methyltransferase Pcm1 in vivo.Molecular and cellular biology · 2006Article
- mRNA capping enzyme activity is coupled to an early transcription elongation.Molecular and cellular biology · 2004Article
- Deletion of individual mRNA capping genes is unexpectedly not lethal to Candida albicans and results in modified mRNA cap structures.Eukaryotic cell · 2002Article
- The RNA capping machinery as an anti-infective target.Wiley interdisciplinary reviews. RNAReview
- Enzymology of RNA cap synthesis.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
The Saccharomyces cerevisiae mRNA capping enzyme consists of two subunits: an RNA 5'-triphosphatase (RTPase) and GTP::mRNA guanylyltransferase (GTase). The GTase subunit (Ceg1) binds to the phosphorylated carboxyl-terminal domain of the largest subunit (CTD-P) of RNA polymerase II (pol II), coupling capping with transcription. Ceg1 bound to the CTD-P is inactive unless allosterically activated by interaction with the RTPase subunit (Cet1). For purposes of comparison, we characterize here the related GTases and RTPases from the yeasts Schizosaccharomyces pombe and Candida albicans. Surprisingly, the S. pombe capping enzyme subunits do not interact with each other. Both can independently interact with CTD-P of pol II, and the GTase is not repressed by CTD-P binding. The S. pombe RTPase gene (pct1+) is essential for viability. Pct1 can replace the S. cerevisiae RTPase when GTase activity is supplied by the S. pombe or mouse enzymes but not by the S. cerevisiae GTase. The C. albicans capping enzyme subunits do interact with each other. However, this interaction is not essential in vivo. Our results reveal an unexpected diversity among the fungal capping machineries.
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Registered trials
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